产品名称
4-硝基苯基 β- D -纤维二糖, ≥98% (TLC)
InChI
1S/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9-,10-,11-,12+,13-,14-,15-,16-,17-,18+/m1/s1
SMILES string
OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O[C@@H]2CO)Oc3ccc(cc3)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O
InChI key
IAYJZWFYUSNIPN-KFRZSCGFSA-N
assay
≥98% (TLC)
form
powder
solubility
water: 49.00-51.00 mg/mL
storage temp.
2-8°C
Quality Level
Application
4-硝基苯基β-D-吡喃木糖苷已用作底物,以测定β-D-纤维二糖苷酶或纤维二糖水解酶(胞外纤维素酶)在微生物群落中的活性。它还可用作底物来研究内切葡聚糖酶J30的活性。
Biochem/physiol Actions
4-硝基苯基 β-D-吡喃木糖苷是一种纤维三糖类似物,和常用于检测纤维素酶活性的显色底物。外切葡聚糖酶、内切葡聚糖酶和β -葡糖苷酶可水解4-硝基苯基 β-D-吡喃木糖苷,以形成p-硝基酚(PNP)。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
涉药品监管产品
此项目有
Keum Shin et al.
Journal of microbiology and biotechnology, 20(12), 1681-1688 (2011-01-05)
A screening for cellobiohydrolase (CBH) activity was performed and Fomitopsis pinicola KMJ812 was selected for further characterization as it produced a high level of CBH activity. An extracellular CBH was purified to homogeneity by sequential chromatography of F. pinicola culture
M Fukumura et al.
Bioscience, biotechnology, and biochemistry, 59(1), 40-46 (1995-01-01)
The nucleotides of the xynB gene of Clostridium stercorarium F-9 were sequenced. The structural gene consists of an open reading frame of 1161 bp encoding a xylanase (XynB) in family F of 387 amino acids with a molecular weight of
K A Taylor et al.
Applied and environmental microbiology, 53(1), 41-46 (1987-01-01)
A cel gene from Bacteroides succinogenes inserted into the vector pUC8 coded for an enzyme which exhibited high hydrolytic activity on carboxymethylcellulose, p-nitrophenylcellobioside, and lichenan and low activity on laminarin and xylan. The enzyme was not synthesized by the Escherichia
A G Day et al.
Biochemical and biophysical research communications, 133(2), 628-632 (1985-12-17)
Unusual kinetic behaviour was observed in assaying spectrophotometrically for exo-glucanase activity in a beta-glucosidase isolated from A. faecalis using p-nitrophenyl beta-cellobioside as substrate. At high substrate concentrations no phenol was released whereas at low concentrations a rapid release of phenol
Kyung-Min Lee et al.
Journal of microbiology and biotechnology, 21(7), 711-718 (2011-07-28)
A highly efficient cellobiohydrolase (CBH)-secreting basidiomycetous fungus, Agaricus arvensis KMJ623, was isolated and identified based on its morphological features and sequence analysis of internal transcribed spacer rDNA. An extracellular CBH was purified to homogeneity from A. arvencis culture supernatant using
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