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Merck
CN

379204

酸性蓝83

Dye content 50 %

别名:

Coomassie 亮蓝R, 亮吲哚蓝蛋白 6B, 酸性蓝 83

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关于此项目

经验公式(希尔记法):
C45H44N3NaO7S2
化学文摘社编号:
分子量:
825.97
EC Number:
228-060-5
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
5718025
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InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

composition

Dye content, 50%

λmax

588 nm

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Application

电泳后检测蛋白条带。

Legal Information

存储类别

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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Yong-Hak Kim et al.
Archives of biochemistry and biophysics, 494(2), 159-165 (2009-12-01)
We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was
G Houen et al.
Electrophoresis, 18(5), 701-705 (1997-05-01)
A method for staining proteins on polyvinylidene difluoride membranes without using organic solvent is described. The method uses preblocking of the membrane with either Tween 20 or polyethylene glycol followed by staining with 0.01% Coomassie Brilliant Blue. No destaining of
G Sreeramulu et al.
Electrophoresis, 16(3), 362-365 (1995-03-01)
A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (<
Recovery of single-band proteins from polyacrylamide gels by using electrophoresis in solutions with different ionic strengths and specific weights.
Ding-Gan Liu
Analytical biochemistry, 339(2), 351-352 (2005-03-31)
Melda Altikatoglu et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 39(3), 185-190 (2010-12-02)
Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However

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