TissueFab^® xeno-free bioink

Rethink your bioinks with TissueFab^®

About the material:

These TissueFab(R) xeno-free bioinks are animal-origin free, tunable, and require no UV, no temperature/pH curing, or chemical crosslinking. This bioink is modified with the bioactive peptide IKVAV to promote cell adhesion, migration, and differentiation.

Printing and crosslinking parameters:

• Compatible with extrusion-based printing
• Ready to use at room temperature *
• Neutral in pH
• Transparent
• Does not require additional functionalization or crosslinking such as methacrylation or UV curing

The TissueFab(R) xeno-free bioinks represent a versatile family of ready-to-use, xeno-free tunable bioink systems. Distinguished by their unique shear-thinning and rapid recovery mechanical properties, these bioinks can uphold the printed structure without the need for UV or other specialized curing methods, making them exceptionally well-suited for cell encapsulation. Furthermore, the introduction of cell culture media post-printing can provide additional stabilization to the printed structure and promote cell growth.

The IKVAV peptide, derived from laminin, a major protein found in the extracellular matrix (ECM) of many tissues, is integral to supporting cell adhesion, migration, and differentiation. This specific peptide fragment within laminin has been identified for its remarkable ability to promote cell-matrix interactions. When incorporated into the TissueFab(R) xeno-free bioink, the IKVAV modification enhances the development and functionality of cell-encapsulated 3D bioprinted tissues. This advancement is particularly valuable in tissue engineering and regenerative medicine applications, where robust cell-matrix interactions are essential for the successful biofabrication of functional tissues.

More information and a step by step protocol can be found under the "More Documents" section of this page.

The TissueFab^® xeno-free bioinks are versatile materials suitable for a range of bioprinting applications, including 3D cell culture, tissue engineering, and regenerative medicine. Their xeno-free (or animal-free) nature makes them ideal for studying complex cell-matrix or cell-cell interactions, while the UV-free bioprinting process promotes excellent cell compatibility for diverse applications, such as 3D cell culture, tissue engineering, drug screening and regenerative medicine. The IKVAV peptide is commonly used in various types of tissue engineering including:

• Neural Tissue Engineering: Supporting the development of biofabricated neural tissues, nerve guidance conduits, and constructs for neural regeneration and repair.
• Musculoskeletal Tissue Engineering: Facilitating the creation of biofabricated musculoskeletal tissues, bone grafts, and scaffolds for bone and muscle regeneration and repair.
• Vascular Tissue Engineering: Enhancing the biofabrication of vascular tissues, blood vessel constructs, and scaffolds for cardiovascular tissue repair and regeneration.
• Skin Tissue Engineering: Supporting the development of biofabricated skin constructs, dermal substitutes, and wound healing scaffolds for skin tissue repair and regeneration.
• Organoid and Organ-on-a-Chip Models: Contributing to the creation of biofabricated organoids and organ-on-a-chip models for studying organ development, disease modeling, and drug testing.

Key Features and Benefits: The TissueFab(R) xeno-free bioinks are:

• Xeno-free, animal-origin free
• Tunable
• Ready to use as room temperature
• Facile curable - no UV, temperature/pH curing, or chemical crosslinking required
• Transparent - excellent visibility after printing and cell culture
• IKVAV modified - to promote cell attachment, spreading and proliferation

In addition, each TissueFab(R) material is developed and tested to meet strict criteria to best suit researchers′ needs. This includes:

• Printability
• Cell viability
• Biocompatibility
• Mechanical and rheological properties

This product contains 3mL of bioink solution.

TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany

VitroINK is a registered trademark of TheWell Bioscience Inc.