Cap A

Name: Cap A
Brand: ALDRICH

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关于此项目

NACRES:

NA.21

UNSPSC Code:

12352200

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属性

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

说明

Preparation Note

乙酸酐/四氢呋喃 9.1/90.9 (v/v)

Other Notes

Cap A solution, containing acetic anhydride and tetrahydrofuran (THF), is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.