Control (2mL vial)

Particle size and shape: Vehicle control mean particle size is 80 to 90 nm, with very small poly-dispersity valueFormulation: The long-circulating, pegylated, liposomal carrier is composed of N-(carbonyl-ethoxypolyethylene glycol 2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine sodium salt (MPEG-DSPE), 3.19 mg/ml; fully hydrogenated soy phosphatidylcholine (HSPC), 9.58 mg/ml; cholesterol, 3.19 mg/ml, ammonium sulfate, approximately 2 mg/ml; histidine as a buffer; hydrochloric acid or sodium hydroxide for pH control; and sucrose to maintain isotonicity. The Control is provided as a sterile, translucent, liposomal dispersion in single-use, 2 ml or 5 ml vials.Buffer: 10 mM histidine buffer, pH 6.5-6.8, with 10% w/v sucrose.

Intravenous (i.v.) injection in flank or orthotopic animal models of cancer.

2 mL Clear Glass Vial with Stopper and Crimp Cap (300103S-1EA)

Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20°C. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.

Store undiluted at 2°-8° C. Do Not Freeze.

Delivery to cells: Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.

Effective concentration: Most biological responses saturate at 20 to 250 μM sn-1,2-dioctanoylglycerol. Only sn-1,2 isomers appear to be active.

Precaution: Since short chain Diacylglycerols mimic effects of the tumor-promoting phorbol diesters in a number of biological systems, extra care should be employed in their handling. Treatment of solutions, vessels and other articles with 1N NaOH before washing or discarding will destroy diacylglycerols.

Avanti Research is a trademark of Avanti Polar Lipids, LLC