产品名称
E06 mAb-biotinylated, E06 Mouse Monoclonal Antibody (IgM), Biotinylated Anti-(Oxidized Phospholipid), 1X TBS (Tris Buffered Saline) with 1%BSA
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
assay
>95%
form
solution
packaging
pkg of 1 × 0.125 mL (polypropylene tube with screw cap (330002S-25ug))
manufacturer/tradename
Avanti Research™ - A Croda Brand 330002S
concentration
0.2 mg/mL (330002S-25ug)
shipped in
dry ice
storage temp.
−70°C
General description
Avanti now provides high purity E06 from an animal-free culture of hybridoma cells. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.
Application
E06 mAb-biotinylated has been used in immunodetection. It may be used in immunohistochemistry, western blot analysis and in in vivo imaging with magnetic resonance imaging (MRI) techniques.
The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 μg/mL working solution of 12 μg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 μg/mL working solution (1:100 dilution).
Biochem/physiol Actions
The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized Low-density lipoprotein (LDL), oxidized High-density lipoprotein (HDL) and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in rabbits, monkeys, mice, zebrafish and humans. It also binds to apoptotic cells, but not viable cells.
Packaging
0.5 mL Polypropylene Tube with Screw Cap (330002S-25ug)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma.
Palinski W, et al.
The Journal of Clinical Investigation, 98(3), 800-814 (1996)
Increased plasma oxidized phospholipid: apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression
Tsimikas S, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 27(1), 175-181 (2007)
Impact of inhibition of the autophagy-lysosomal pathway on biomolecules carbonylation and proteome regulation in rat cardiac cells
Coliva G, et al.
Redox Biology, 23, 101123-101123 (2019)
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