330021S
Avanti
WR304 mAb
Avanti Research™ - A Croda Brand
别名:
WR304 mAb, WR304 Mouse Monoclonal Antibody (IgM), Anti-(PIP/PIP2), 1X TBS (Tris Buffered Saline) with 1mM Na2 EDTA
产品名称
WR304 mAb, Avanti Research™ - A Croda Brand 330021S
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
方案
>95%
包装
pkg of 1 × 0.1 mL (polypropylene tube with screw cap (330021S-100ug))
制造商/商品名称
Avanti Research™ - A Croda Brand 330021S
浓度
1 mg/mL (330021S-100ug)
运输
dry ice
储存温度
−70°C
一般描述
WR304 is an antibody that binds specifically to the lipids PIP and PIP2. WR304 also neutralizes infectious HIV-1 virus and can be used to probe the presence of PIP and PIP2 in membranes in a variety of applications including inositol phosphate-dependent cell signaling pathways. This antibody was developed by the Walter Reed Army Institute of Research (WRAIR) using a targeted adjuvant protocol with liposomes containing Avanti′s Lipid A.
Formulation: WR304 is provided as a 0.2μm sterile-filtered solution in Tris buffered saline (TBS). WR304 binding to porcine brain PI(4)P is inhibited by high concentration of Ca2+ (10 mM)iv and addition of 1 mM EDTA stimulates this binding activity. PBS and other phosphate-containing buffers should not be used as they inhibit binding to the phospholipid head groupiii.
Formulation: WR304 is provided as a 0.2μm sterile-filtered solution in Tris buffered saline (TBS). WR304 binding to porcine brain PI(4)P is inhibited by high concentration of Ca2+ (10 mM)iv and addition of 1 mM EDTA stimulates this binding activity. PBS and other phosphate-containing buffers should not be used as they inhibit binding to the phospholipid head groupiii.
免疫原
Antigens: Brain PI(4)P
应用
ELISA, IHC, Dot Blot, Flow cytometry and HIV-1 infectivity.
The WR304 antibody has been used for the quantitation of PIP/PIP2 in direct ELISA and Dot Blot analysis. The WR304 antibody specifically binds to PIP/PIP2 and inhibits the infectivity of HIV-1 to peripheral blood mononuclear cells (PBMC).
The WR304 antibody has been used for the quantitation of PIP/PIP2 in direct ELISA and Dot Blot analysis. The WR304 antibody specifically binds to PIP/PIP2 and inhibits the infectivity of HIV-1 to peripheral blood mononuclear cells (PBMC).
生化/生理作用
WR304 recognizes PIP and PIP2, and neutralizes HIV-1 virus.
包装
0.5 mL Polypropylene Tube with Screw Cap (330021S-100ug)
制备说明
Store undiluted at either -20°C or -80°C.
其他说明
Hazardous/Non-hazardous Components: This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore, there is no MSDS for this product.
Ig Class: Mouse IgM (kappa).
Antibody Source: Monoclonal antibody from BALB/c-derived hybridoma WR304.
Production: In vitro cell culture. Purification: Ultra filtration through 100 KDa cut-off filters.
Purification: Ultra filtration through 100 KDa cut-off filters.
Antibody Source: Monoclonal antibody from BALB/c-derived hybridoma WR304.
Production: In vitro cell culture. Purification: Ultra filtration through 100 KDa cut-off filters.
Purification: Ultra filtration through 100 KDa cut-off filters.
法律信息
Avanti Research is a trademark of Avanti Polar Lipids, LLC
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
N M Wassef et al.
Molecular immunology, 21(10), 863-868 (1984-10-01)
Monoclonal antibodies against phosphatidylinositol phosphate were produced after injecting a mouse with liposomes containing dimyristoylphosphatidylcholine, cholesterol, phosphatidylinositol phosphate and lipid A. The antibodies raised were IgM (kappa) and their activities were assayed by complement-dependent damage to liposomes lacking lipid A
Bruce K Brown et al.
Journal of virology, 81(4), 2087-2091 (2006-12-08)
Both a murine monoclonal antibody to phosphatidylinositol phosphate (PIP) and a human monoclonal antibody (4E10) that is known to have broadly neutralizing capabilities against primary isolates of human immunodeficiency virus type 1 (HIV-1) bound to PIP, as determined by enzyme-linked
Gary R Matyas et al.
Biochimica et biophysica acta, 1788(3), 660-665 (2008-12-23)
Two human mAbs (2F5 and 4E10), originally derived from HIV-1-infected patients, are important, but rare, mAbs that exhibit broad cross-clade neutralizing activities against HIV-1. In addition to peptide sequences on the gp41 envelope protein, both antibodies reportedly also bound specifically
Zoltan Beck et al.
Biochemical and biophysical research communications, 354(3), 747-751 (2007-01-30)
The binding characteristics of two monoclonal antibodies (mAb) to phosphatidylinositol-4-phosphate (PIP) were examined: a murine IgM mAb to PIP; and a human IgG mAb (4E10) that binds both to HIV-1 envelope protein and also to neutral and anionic phospholipids, including
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