Cer1P ELISA (Clear)

Avanti Lipid Snoopers^™ are lipid microarrays useful in semi-quantitative measurements. These snoopers are relatively stable and provide a higher sensitivity compared to the conventional enzyme-linked immunosorbent assays (ELISA) methods. Ceramide-1-Phosphate (Cer1P) is a bioactive anionic sphingolipid. Ceramide kinase (CERK) mediates the synthesis of Cer1P. Cer1P is also synthesized by the hydrolysis of sphingomyelin in the presence of enzyme sphingomyelinase (SMase).

ELISA Snoopers^™ are 8-well strips pre-coated with a single high-quality lipid species. They can be used to investigate protein-lipid interactions, and characterize and titrate lipid binding proteins. This format provides the following advantages:

• quantitative lipid-protein binding studies
• assemble a 96-well plate using only the lipids of interest for binding studies
• flexibility - use 8 to 96 wells, and 1 to 12 lipids as needed.

The 8-well strips are available in optically clear, white, or black surfaces for use in colormetric, luminescent and fluorescent assays, respectively. Avanti Lipid Snoopers ELISA Strips allow multiple sample conditions to be probed simultaneously in a single plate making them a perfect complement to Avanti Lipid Snoopers Nitrocellulose Strips.

Ceramide-1-Phosphate (Cer1P) mediates macrophage migration, regulates autocrine and paracrine mediators. Elevated levels of Cer1P leads to negative regulation of ceramide kinase (CERK). The intracellular calcium levels modulate Cer1P and CERK functionality. Cer1P is also a crucial regulator of phagocytosis, inflammation, apoptosis and mitosis. Avanti Lipid Snoopers^™ have been used successfully in elucidating the role of annexin V in membrane trafficking.

ELISA Strip Packaged in Foil Pouch (330687-1EA)

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Snoopers is a trademark of Avanti Polar Lipids, LLC