方案
>99% (MS)
表单
powder
制造商/商品名称
Avanti Research™ - A Croda Brand
包装
1 ea of 1 × (with stopper and crimp cap)
运输
dry ice
储存温度
−20°C
一般描述
Avanti′s new HSA lipid delivery system, Huzzah™ improves the cellular delivery of S1P through its conjugation with human serum albumin (HSA), a physiologically relevant carrier protein. This conjugate system eliminates the need for organic solvents, which are well-known to adversely affect viability and the phenotypic characteristics of cells. The Huzzah™ lipid delivery system is supplied as a lyophilized conjugate which can be directly and easily reconstituted in water or a buffer of choice and is ideal for studying the effects of poorly soluble bioactive molecules. Solubility: Reconstitute lyophilized conjugate in 5 mL of water or other aqueous buffer. This produces a stock solution of 120 - 200 μM sphingosine-1-phosphate*. A short sonication may be necessary to ensure complete dissolution. If necessary, filter sterilize the reconstituted conjugate prior to use in cell culture applications. *Exact concentration will vary from batch to batch. Refer to label for exact product concentration upon reconstitution as determined by mass spectrometric analysis.Molar Ratio: S1P:albumin (2:1) Albumin Biological Characterization: microbial free; endotoxin < 1 IU/mL Albumin Lipid Characterization: ~0.07g/L phospholipids; ~0.02 g/L sphingolipids; ~0.1 g/L fatty acids; and triglycerides and cholesterols below the limit of quantitation of 0.1 g/L and 0.05 g/L.
应用
Huzzah™ S1P has been used:
- to study its role in ovarian cancer (OvCa) tumor progression
- for the differentiation of mouse embryonic stem (ES) cells toward neuronal and glial lineage
- to deliver aqueous soluble lipid species to cells
包装
10 mL Clear Glass Vial with Stopper and Crimp Cap (360492P-1EA)
法律信息
Avanti Research is a trademark of Avanti Polar Lipids, LLC
Huzzah is a trademark of Avanti Polar Lipids, LLC
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品--人源产品
此项目有
SPHK1 is a novel target of metformin in ovarian cancer
Hart PC, et al.
Molecular Cancer Research (2019)
Ilaria Del Gaudio et al.
International journal of molecular sciences, 21(3) (2020-01-30)
Placental inflammation and dysfunction during pregnancy are associated with short- and long-term adverse outcomes for the offspring. However, the mechanisms of vascular protection at the feto-placental interface are still poorly investigated. The high-density lipoprotein (HDL) associated sphingosine-1-phosphate (S1P) has been
Sphingosine-1-Phosphate Signaling in Immunology and Infectious Diseases (2018)
Ilaria Del Gaudio et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865(4), 158632-158632 (2020-01-19)
Perinatal and long-term offspring morbidities are strongly dependent on the preservation of placental vascular homeostasis during pregnancy. In adults, the HDL-apoM-S1P complex protects the endothelium and maintains vascular integrity. However, the metabolism and biology of cord blood-derived HDLs (referred to
Avanti lipid tools: connecting lipids, technology, and cell biology
Sims KH, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1841(8), 1038-1048 (2014)
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