产品名称
16:0-14 Doxyl PC, Avanti Research™ - A Croda Brand 810605C
assay
>99% (TLC)
form
liquid
packaging
pkg of 1 × 1 mL (810605C-1mg)
manufacturer/tradename
Avanti Research™ - A Croda Brand 810605C
concentration
1 mg/mL (810605C-1mg)
shipped in
dry ice
storage temp.
−20°C
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General description
Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.
Application
16:0-14 Doxyl PC has been used:
- as a spin label for electron paramagnetic resonance (EPR) studies
- in labeling of small unilamellar vesicles (SUV) for electron spin resonance (ESR) studies
- in polarity profile studies of phospholipid membranes by high-frequency ESR
Biochem/physiol Actions
16:0-14 Doxyl phosphatidylcholine (PC) is used in the labeling of small unilamellar vesicles (SUVs) to probe the membrane depth from the hydrocarbon-water interface. n-Doxyl PC is incorporated in membranes for depth quenching measurements. It is used in fluorescence based vesicle quenching studies.
Packaging
5 mL Clear Glass Sealed Ampule (810605C-1mg)
Preparation Note
Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
target_organs
Central nervous system
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
新产品
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Spin-label saturation-recovery EPR at W-band: applications to eye lens lipid membranes
Mainali L, et al.
Journal of Magnetic Resonance, 212(1), 86-94 (2011)
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Dzikovski B, et al.
The Journal of Physical Chemistry B, 116(23), 6694-6706 (2012)
Basis for selectivity of cationic antimicrobial peptides for bacterial versus mammalian membranes
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The Journal of Biological Chemistry, 280(40), 33960-33967 (2005)
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Kamp, et al.
Test, 281(14), 9251-9259 (2006)
Ultra-stable temperature control in EPR experiments: thermodynamics of gel-to-liquid phase transition in spin-labeled phospholipid bilayers and bilayer perturbations by spin labels
Alaouie AM, et. al.
Journal of Magnetic Resonance, 182, 229-238 (2006)
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