SMC^® Human Tumor Necrosis Factor Alpha (TNF-α) Immunoassay Kit

The SMC^® Human TNF-α Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine with cytotoxic activity that is primarily produced by macrophages. TNF-α is involved in systemic inflammation and is a member of the group of cytokines that stimulate the acute phase reaction. When administered to animals or humans it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation, and acute phase responses similar to those seen during acute infections and shock states. By promoting the inflammatory response, TNF-α is associated with many autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn′s disease, psoriasis, and refractory asthma. TNF-α also plays a role in cancer and regulates apoptotic cell death, cellular proliferation, differentiation, tumorigenesis, and viral replication.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

The sensitivity of Single Molecule Counting (SMC^®) technology gives the SMC^® Human TNF-α Immunoassay Kit unparalleled performance in the quantification of TNF-α in human EDTA plasma.

Used to detect/quantify:TNF-α

Low-end CV% Range: 5 - 9%|Low-end CV% Average: 7%

TNF-α Coated Beads
Standard Diluent
TNF-α Detection Antibody
Assay Buffer
Human TNF-α Standard
10X Wash Buffer
Elution Buffer B
Buffer D
SMC^® Human TNF-α Immunoassay Kit Instructions

SMC is a registered trademark of Singulex

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial