Anti-IRF4 Antibody, clone 3E4

Interferon regulatory factor 4, also known as IRF4, belongs to the IRF family. IRF4 encodes a 450 amino acid polypeptide with a predicted molecular weight of 51.6 kDa (Grossman 1996). The IRF4 gene is expressed in the spleen, lymphocytes and melanocytes, and is induced in to T cells after cross linking with the T cell receptor. IRF4 is a transcriptional activator that binds to the interferon stimulated response element (ISRE) of the MHC class I promoter. IRF4 is essential for mature T and B lymphocyte function. IRF4 interacts with MYD88 to prevent the interaction of IRF5, resulting in negative regulation of Toll Like Receptor (TLR) signaling. High levels of IRF4 have been associated with Epstein Barr Virus (EBV) and may be critical to EBV transformation. Further oncogenic potential has been associated with the involvement of IRF4 with hair color and pigmentation of the skin, which may cause multiple myeloma.

~51 kDa observed

GST-tagged recombinant protein corresponding to the C-terminus of mouse IRF4.

Anti-IRF4 Antibody, clone 3E4 is a highly specific rat monoclonal antibody, that targets IRF4 & has been tested in western blotting, IHC & Flow Cytometry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected IRF4 in positive human tonsil tissues.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected IRF4 in wildtype and IRF4 knockout mice (Zheng, Y., et al. (2009). Nature. 458(7236):351-356.).

Research Category
Inflammation & Immunology

Research Sub Category
Transcription Factors

Format: Purified

Protein G Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected IRF4 in 10 µg of Ramos cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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