04-1570
抗RNA聚合酶II亚基B1（磷酸化CTD Ser-7）抗体，克隆4E12
clone 4E12, from rat
别名:
DNA-directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1, RNA-directed RNA
产品名称
抗RNA聚合酶II亚基B1（磷酸化CTD Ser-7）抗体，克隆4E12, clone 4E12, from rat
biological source
rat
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4E12, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
ChIP: suitable
ELISA: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer7)
Quality Level
Gene Information
human ... POLR2B(5431)
Analysis Note
对照
γ-蛋白磷酸酶（γ-PPase）未处理和处理的NIH/3T3细胞裂解液
γ-蛋白磷酸酶（γ-PPase）未处理和处理的NIH/3T3细胞裂解液
已通过蛋白质印迹在未经处理和经γ-PPase处理的NIH/3T3细胞裂解液中进行了评估。
蛋白质印迹分析：0.25 µg/ml该抗体在10 µg γ-PPase未处理和处理的NIH/3T3细胞裂解液上检测到RNA聚合酶II CTD。
蛋白质印迹分析：0.25 µg/ml该抗体在10 µg γ-PPase未处理和处理的NIH/3T3细胞裂解液上检测到RNA聚合酶II CTD。
Application
抗RNA聚合酶II亚基B1（磷酸化-CTD Ser-7）抗体，克隆4E12是用于检测RNA聚合酶II亚基B1（磷酸化-CTD Ser-7）的大鼠单克隆抗体，已在WB，ELISA中进行了验证。
染色质免疫沉淀分析： 代表性批次已被独立实验室用于ChIP。(Chapman, R., et al. (2007).Science.318(5857):1780 -1782.)
研究子类别
转录因子
RNA代谢 & 结合蛋白
转录因子
RNA代谢 & 结合蛋白
研究类别
表观遗传学 & 核功能
表观遗传学 & 核功能
表观遗传学 & 核功能
表观遗传学 & 核功能
Biochem/physiol Actions
该抗体可识别CTD处Ser7磷酸化的RNA聚合酶II亚基B1。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
RNA聚合酶II亚基B1（RPB1）是RNA聚合酶II复合物中最大的亚基。作为全酶RNA聚合酶II使用四种核糖核苷三磷酸作为底物，催化真核DNA转录为RNA。RBP1亚基与其他聚合酶亚基结合形成一个大的中央裂口，该裂口保持酶的活性位点，DNA模板和新生的RNA转录本之间的接触。该亚基还包含由串联七肽重复序列组成的羧基末端结构域（CTD）。磷酸化激活RNA聚合酶IIβ亚基，使其可以作为调节起始，延伸，终止和mRNA加工的其他亚基的组装平台。在主动转录RNA聚合酶中，七肽重复序列的‘Ser-2’和‘Ser-5’被磷酸化。Ser-7在启动子区域开始转录之前被磷酸化。
~220 kDa
Immunogen
与Ser7磷酸化的人RNA聚合酶亚基B1 CTD对应的卵清蛋白偶联线性肽。
表位：Ser7
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
形式：纯化
纯化的大鼠单克隆IgG1κ溶于含0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl和0.05％叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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