04-1624
抗-Centrin抗体，克隆20H5
clone 20H5, from mouse
别名:
Caltractin isoform 2, EF-hand protein, calcium binding protein, centrin 1, centrin, EF-hand protein, 1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
20H5, monoclonal
Application:
ICC, IP, WB
Citations:
198
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
20H5, monoclonal
species reactivity
rat, human, mouse
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CETN1(1068)
General description
~20 kDa
中心体蛋白是中心粒复制的关键调节因子。该蛋白质位于相间细胞的中心体。在有丝分裂期间，中心蛋白重新分布到纺锤极，这与细胞周期中中心体的动态变化一致。当前的研究表明，中心蛋白通过其作为中心粒前体结构的组成部分的作用以及通过其丰度的周期性细胞周期特异性变化来发挥对中心粒复制的空间和时间控制。
Immunogen
对应于衣藻中心体蛋白的C端的重组蛋白。
表位：C端
Application
免疫细胞化学分析： Manandhar, G., et al. (1999).Cell Motil Cytoskeleton.43(2):137-144.
抗中心蛋白抗体，克隆20H5是用于检测中心蛋白的小鼠单克隆抗体，也称为钙调素亚型2，EF-手蛋白&已在WB、IP、ICC中得到验证。
研究子类别
细胞周期，DNA 复制&修复
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
该抗体识别中心体蛋白的C端。
Physical form
形式：纯化
纯化的小鼠单克隆IgG2aκ，溶于含有0.1 M Tris-甘氨酸（pH 7.4，150 mM NaCl）和0.05％叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
Analysis Note
对照
HeLa细胞裂解液
HeLa细胞裂解液
通过HeLa细胞裂解液中的蛋白质印迹进行了评估。
蛋白质印迹分析：1 µg/ml该抗体可在10 µg的HeLa细胞裂解液中检测到中心体蛋白。
蛋白质印迹分析：1 µg/ml该抗体可在10 µg的HeLa细胞裂解液中检测到中心体蛋白。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
G Manandhar et al.
Cell motility and the cytoskeleton, 43(2), 137-144 (1999-06-24)
Centrosome reduction during mouse spermiogenesis has been studied by immunofluorescent microscopy using anticentrin antibody (20H5) and TEM. Centrin is detected as two spots in round spermatids, corresponding to a pair of centrioles. In elongating spermatids, centrin spots colocalize with the
Clare R Harding et al.
PLoS pathogens, 12(2), e1005403-e1005403 (2016-02-06)
The inner membrane complex (IMC) of apicomplexan parasites is a specialised structure localised beneath the parasite's plasma membrane, and is important for parasite stability and intracellular replication. Furthermore, it serves as an anchor for the myosin A motor complex, termed
Rachael A McCloy et al.
Cell cycle (Georgetown, Tex.), 13(9), 1400-1412 (2014-03-15)
Entry and progression through mitosis has traditionally been linked directly to the activity of cyclin-dependent kinase 1 (Cdk1). In this study we utilized low doses of the Cdk1-specific inhibitor, RO3306 from early G 2 phase onwards. Addition of low doses
Mi Young Seo et al.
PloS one, 10(9), e0138905-e0138905 (2015-09-26)
A procentriole is assembled next to the mother centriole during S phase and remains associated until M phase. After functioning as a spindle pole during mitosis, the mother centriole and procentriole are separated at the end of mitosis. A close
Stress-induced localization of HSPA6 (HSP70B') and HSPA1A (HSP70-1) proteins to centrioles in human neuronal cells.
Khalouei, S; Chow, AM; Brown, IR
Cell Stress & Chaperones null
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