biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
24B5, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ERBB2(2064)
mouse ... Erbb2(13866)
General description
185 kDa
Immunogen
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of the intracellular domain in human erbB2
Application
Anti-erbB2 (intracellular domain) Antibody, clone 24B5 detects level of erbB2 (intracellular domain) & has been published & validated for use in ELISA & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
Recognizes the intracellular domain of erbB2 (aa 860-880).
Physical form
Format: Purified
Subsequent thiophilic adsorption and size exclusion chromatography
lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Preparation Note
2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
EGF treated SKOV-3 lysates
EGF treated SKOV-3 lysates
Routinely evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
H S Wiley et al.
The Journal of cell biology, 143(5), 1317-1328 (1998-12-02)
Autocrine EGF-receptor (EGFR) ligands are normally made as membrane-anchored precursors that are proteolytically processed to yield mature, soluble peptides. To explore the function of the membrane-anchoring domain of EGF, we expressed artificial EGF genes either with or without this structure
Stephen J Collins et al.
Chemical biology & drug design, 101(1), 87-102 (2022-08-28)
Human epidermal growth factor receptor 2 (HER2) is an oncogenic driver and key therapeutic target for human cancers. Current therapies targeting HER2 are primarily based on overexpression of the wild-type form of HER2. However, kinase domain mutations have been identified
Expression and purification of active human kinases using Pichia pastoris as a general-purpose host.
May H Abdel Aziz et al.
Protein expression and purification, 179, 105780-105780 (2020-10-30)
The heterologous expression of human kinases in good purity and in a monomeric, soluble and active form can be challenging. Most of the reported successful attempts are carried out in insect cells as a host. The use of E. coli
Tiffany Tsui et al.
Biochemistry, 54(20), 3173-3182 (2015-05-06)
Brk (breast tumor kinase, also known as PTK6) is a nonreceptor tyrosine kinase that is aberrantly expressed in several cancers and promotes cell proliferation and transformation. Genome sequencing studies have revealed a number of cancer-associated somatic mutations in the Brk
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