Anti-c-Jun Antibody, clone 6E4.4

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

This antibody detects c-Jun, regardless of phosphorylation state.

GST-tagged recombinant protein corresponding to c-Jun.

Anti-c-Jun Antibody, clone 6E4.4 is a Mouse Monoclonal Antibody for detection of c-Jun also known as JNK1 alpha protein kinase, JNK1 beta protein kinase & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western Blot Analysis (SNAP ID Technique): 5 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate using the SNAP ID Tecnique).

Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2).

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate.

~36-43 kDa
Multiple bands may be visible, indicating various phosphorylation states.

Replaces: 04-210

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.

Stable at 2-8°C for 1 year from date of receipt.

Control
NIH/3T3 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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