05-1417
Anti-PTP-PEST Antibody, clone AG25
ascites fluid, clone AG25, from mouse
别名:
Protein-tyrosine phosphatase G1, Tyrosine-protein phosphatase non-receptor type12, Protein tyrosine phosphatase, non-receptor type 12
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
AG25, monoclonal
Application:
ICC, IP, WB
Citations:
1
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
AG25, monoclonal
species reactivity
human, mouse, rat
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PTPN12(5782)
General description
The protein tyrosine phosphatase PTP-PEST is an cytosolic protein tyrosine phosphatase that is ubiquitously expressed in mammalian tissues. PTP-PEST associates with multiple cytoskeleton and cell adhesion molecules suggesting a regulatory role in controlling cell shape and motility.
~88 kDa
Immunogen
Baculovirus expressed purified human PTP-PEST
Application
Anti-PTP-PEST Antibody, clone AG25 detects level of PTP-PEST & has been published & validated for use in WB, IP & IC.
Immunoprecipitation: Recommended.
Immunocytochemistry: Recommended.
Immunocytochemistry: Recommended.
Biochem/physiol Actions
Recognizes human PTP-PEST.
Physical form
Mouse monoclonal IgG1 ascites containing no preservatives.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PTP-PEST on 10 μg of HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PTP-PEST on 10 μg of HeLa lysates.
Other Notes
Replaces: MAB3739
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Rima Chattopadhyay et al.
Redox biology, 12, 438-455 (2017-03-21)
Resolvins are a novel class of lipid mediators that play an important role in the resolution of inflammation, although the underlying mechanisms are not very clear. To explore the anti-inflammatory mechanisms of resolvins, we have studied the effects of resolvin
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