biological source
mouse
antibody product type
primary antibodies
clone
9H4.2, monoclonal
species reactivity
mouse, rat, human
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgMκ
NCBI accession no.
UniProt accession no.
Quality Level
Gene Information
human ... PIK3C2A(5286)
General description
PI3 Kinase, class II a (PI3KC2A) is also known as phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha. It plays an important role in vesicular transport in eukaryotic cells. PI3 Kinase-C2-alpha also plays a part in cellular proliferation and survival.
~190 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to human PI3K-C2-alpha.
Application
Anti-PI3 Kinase-C2-alpha Antibody, clone 9H4.2 detects level of PI3 Kinase-C2-alpha & has been published & validated for use in WB & IC.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected PI3K-C2-alpha in A431 cells.
Research Category
Signaling
Signaling
Research Sub Category
Kinases & Phosphatases
Kinases & Phosphatases
Physical form
Format: Purified
Ion Exchange
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Jurkat cell lysate
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PI3K-C2-alpha on 10 µg of Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PI3K-C2-alpha on 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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