05-1572
抗-MDC1抗体（克隆P2B11）
clone P2B11, from mouse
别名:
Nuclear factor with BRCT domains 1, homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint 1
产品名称
抗-MDC1抗体（克隆P2B11）, clone P2B11, from mouse
Quality Level
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P2B11, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
chimpanzee (based on 100% sequence homology), bovine (based on 100% sequence homology), human (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MDC1(9656)
mouse ... Mdc1(240087)
Application
免疫细胞化学分析： 一个代表性批次的1：500稀释液在NIH/3T3细胞中检测到MDC1。
蛋白质印迹分析： 一个代表性批次已被独立实验室用于WB。(Lou, Z., et al. (2003).Nature.421(6926):957-961.)
蛋白质印迹分析： 一个代表性批次已被独立实验室用于WB。(Lou, Z., et al. (2003).Nature.421(6926):957-961.)
抗-MDC1抗体（克隆 P2B11）是用于检测 MDC1（也称为带BRCT结构域1的核因子）的小鼠单克隆抗体，&已在 ICC、& WB 中得到验证。
Biochem/physiol Actions
该抗体识别N末端及周围的MDC1 。
General description
MDC1是粘连蛋白复合物的亚基，它是有丝分裂和减数分裂中姐妹染色单体粘连（sister chromatid cohesion）所需的关键蛋白。该蛋白的温度敏感性突变与姐妹染色单体粘连、染色体凝集和染色体分离的缺陷有关。 其表达受细胞周期调节，在S期达到最高。 MDC1的表达是响应DNA损伤而出现的S- 和 G2/M检查点介导的细胞周期阻滞所必需的。 研究显示MDC1可与DNA双链断裂位点附近的H2A.X 相互作用，并将ATM激酶募集至DNA的损伤区。与这种作用一致的是，MDC1 似乎调节其它涉及DNA损伤检查点的蛋白（如BRCA1和 Chk2）的功能。
~ 184 kDa 由于剪接作用人MDC1 有4种亚型 分子量大约为227、198、196和117 kDa
Immunogen
GST标记的重组蛋白，对应于N-末端及周围的小鼠MDC1。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
形式：纯化
纯化的小鼠单克隆IgG1κ溶于含0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl，不含0.05％叠氮化钠的缓冲液中。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Alexandra Mayer et al.
Cell cycle (Georgetown, Tex.), 15(4), 546-558 (2016-01-09)
Because low levels of DNA double strand breaks (DSBs) appear not to activate the ATM-mediated prophase I checkpoint in full-grown oocytes, there may exist mechanisms to protect chromosome integrity during meiotic maturation. Using live imaging we demonstrate that low levels
Kuntian Luo et al.
Genes & development, 30(23), 2581-2595 (2016-12-13)
Homologous recombination (HR) is one of the major DNA double-strand break (DSB) repair pathways in mammalian cells. Defects in HR trigger genomic instability and result in cancer predisposition. The defining step of HR is homologous strand exchange directed by the
Hung-Sheng Shang et al.
Oncology reports, 36(4), 2207-2215 (2016-08-09)
Cervical cancer is one of the most common cancers in women worldwide and it is a prominent cause of cancer mortality. Curcumin is one of the major compounds from Turmeric and has been shown to induce cytotoxic cell death in
Yung-Luen Shih et al.
Oncology reports, 36(4), 2094-2100 (2016-08-31)
Casticin, a polymethoxyflavone, has been demonstrated to possess anticancer activities, yet no study has shown in detail that casticin induces DNA damage in lung cancer cells. The purpose of this study was to investigate the possible molecular mechanisms of casticin
Yunhui Li et al.
Nature communications, 8, 15752-15752 (2017-06-02)
BRCA1 regulates multiple cellular pathways that maintain genomic stability including cell cycle checkpoints, DNA repair, protein ubiquitination, chromatin remodelling, transcriptional regulation and apoptosis. Receptor-associated protein 80 (RAP80) helps recruit BRCA1 to double-strand breaks (DSBs) through the scaffold protein CCDC98 (Abraxas)
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