Anti-Pax5 Antibody, clone 1H9

Pax5, also known as B-cell-specific transcription factor (BASP), and encoded by the gene Pax5/Pax-5, plays a role in B cell differentiation and neural development and spermatogenesis. Pax5 regulates the expression of CD19 a B lymphoid specific target gene. Additionally, Pax5 interacts with ETS1 and alters its DNA binding abilities and it interacts with DAXX and TLE4. Pax5 is localized to the nucleus. Pax5 is expressed at early B-cell differentiation and in the developing CNS during development. In the adult it is expressed in the testis. Pax5 is also involved in B-cell related diseases and lymphomas and most recently was shown to be the controlling factor of the “Z” protein BZLF1 which controls the latent to lytic switch of Epstein-Barr virus (EBV) in humans.

~42 kDa observed

GST-tagged recombinant protein corresponding to mouse Pax5.

Detect PAX5 using this rat monoclonal antibody, Anti-Pax5 Antibody, clone 1H9 validated for use in western blotting & IHC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pax5 in 10 µg of mouse E13-E14 brain tissue lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Isocitrate dehydrogenase in human tonsil and mouse hindbrain tissue.

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pax5 in 10 µg of Ramos cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MABE517

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