Anti-LacI Antibody, clone 9A5

The lactose operon repressor binds to a regulatory element, an operator gene, to form a genetic switch, the lac operon. This switch negatively regulates the expression of structural genes that follow the site of the operator gene. Inducers may bind to the repressor to reduce or eliminate the affinity of the repressor for its operator, and thereby allow for transcription of respective structural genes. Conversely, other effector molecules may increase the binding affinity of the repressor for its operator and thereby inhibit gene transcription. The inducer or effector molecules that associate with a repressor serve as a chemical signal, which monitors the metabolic state of protein(s) expressed by their respective genes.

~38 kDa observed

TrpE fused with full-length E. Coli LacI.

Anti-LacI Antibody, clone 9A5 is an antibody against LacI for use in Western Blotting.

Research Category
Secondary & Control Antibodies

Research Sub Category
Epitope Tags

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide (This antibody is purified from supernatent.).

Stable for 1 year at 2-8°C from date of receipt.

Control
Rosetta BL21 (DE3) competent E. Coli cell lysate.

Evaluated by Western Blot in Rosetta BL21 (DE3) E. Coli cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected LacI in 10 µg of Rosetta BL21 (DE3) E. Coli cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.