Anti-Cdc25C Antibody, clone TC-15

58kDa

The activity of cyclin-dependent kinases is regulated by their phosphorylation status, which is controlled by the antagonistic action of CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25C is a ~56 kDa dual specific protein phosphatase that controls entry into mitosis by regulating the dephosphorylation of the Cdk1/cyclin B complex. Phosphorylation of Cdc25C creates a binding site for 14-3-3 protein which inhibits Cdc25C. This prevents activation of the Cdk1/cyclin B complex and prevents mitotic entry. Cdc25C is phosphorylated by checkpoint kinases throughout interphase but becomes dephosphorylated during mitosis. Cdc25C is also regulated by p53 via a p53 response element in its promoter, and it is predominantly expressed in the G2 phase of the cell cycle.

GST fusion-protein corresponding to residues 1-258 of human cdc25C. Epitope mapped to residues 222-246.

Detect Cdc25C with Anti-Cdc25C Antibody, clone TC-15 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

cdc25C

Protein G Chromatography

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Format: Purified

2 years at -20°C

Control
HeLa nuclear extract

routinely evaluated by immunoblot on HeLa nuclear extract

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