05-584
抗-Tie2/TEK抗体，克隆Ab33
clone Ab33, Upstate®, from mouse
别名:
CD202b antigen, TEK tyrosine kinase, endothelial, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, venous malformations, multip
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
Ab33, monoclonal
Application:
immunohistochemistry
immunoprecipitation (IP)
western blot
immunoprecipitation (IP)
western blot
Species reactivity:
human, rat, pig, mouse
Citations:
36
Technique(s):
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗-Tie2/TEK抗体，克隆Ab33, clone Ab33, Upstate®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Ab33, monoclonal
species reactivity
human, rat, pig, mouse
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TEK(7010)
Analysis Note
已通过蛋白质印迹对HUVEC细胞的RIPA裂解液进行了评估。
蛋白质印迹分析：0.2-1 μg/mL该抗体在来自HUVEC细胞的RIPA裂解液中检测到Tie2/TEK。
蛋白质印迹分析：0.2-1 μg/mL该抗体在来自HUVEC细胞的RIPA裂解液中检测到Tie2/TEK。
Application
免疫沉淀：根据独立实验室的报道，该抗体可免疫沉淀Tie2/Tek。
免疫组化：报告在经Triton处理的冷冻切片中检测到Tie2/Tek。
此抗体不适用于石蜡包埋切片。
免疫组化：报告在经Triton处理的冷冻切片中检测到Tie2/Tek。
此抗体不适用于石蜡包埋切片。
该抗Tie2/TEK抗体，克隆Ab33已验证可用于 WB、IP、IH中的Tie2/TEK检测。
Biochem/physiol Actions
识别Tie2/TEK，Mr 145kDa
General description
TIE2（具有Ig和EGF同源结构域2的酪氨酸激酶）几乎仅在小鼠，大鼠和人的内皮细胞中表达。该受体具有独特的细胞外结构域，该结构域包含两个免疫球蛋白样环，这些环被三个表皮生长因子样重复序列隔开，这些重复序列与三个纤连蛋白III型重复序列相连。受体的配体是血管生成素1。TIE2的缺陷与遗传性静脉畸形有关。TIE2信号通路似乎对于静脉形态发生中的内皮细胞-平滑肌细胞通讯至关重要。
145 kDa
Immunogen
对应于人Tie2/TEK残基1-745的融合蛋白，具有从感染的SF-9细胞纯化的C端His6-标签。克隆Ab33
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
形式：纯化
纯化的小鼠单克隆IgG1κ，添加甘油至30％之前，溶于含有0.1M Tris-甘氨酸、0.15M NaCl、0.05％叠氮化钠的缓冲液（pH 7.4）中。在-20°C为液体形式。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
Daisuke Sakai et al.
JOR spine, 1(2), e1018-e1018 (2018-06-27)
Recently, Tie2/TEK receptor tyrosine kinase (Tie2 or syn. angiopoietin-1 receptor) positive nucleus pulposus progenitor cells were detected in human, cattle, and mouse. These cells show remarkable multilineage differentiation capacity and direct correlation with intervertebral disc (IVD) degeneration and are therefore
Increased Expression of Angiopoietins and Tie2 in the Lungs of Chronic Asthmatic Mice.
Makinde TO, Agrawal DK
American Journal of Respiratory Cell and Molecular Biology null
Haixia Gong et al.
Scientific reports, 7, 42127-42127 (2017-02-16)
Human endothelial cells (ECs) are widely used to study mechanisms of angiogenesis, inflammation, and endothelial permeability. Targeted gene disruption induced by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-Associated Protein 9 (Cas9) nuclease gene editing is potentially an important tool for
Antiangiogenic gene therapy targeting the endothelium-specific receptor tyrosine kinase Tie2.
Lin, P, et al.
Proceedings of the National Academy of Sciences of the USA, 95, 8829-8834 (1998)
Mary Anna Venneri et al.
Blood, 109(12), 5276-5285 (2007-03-01)
Tumor-infiltrating myeloid cells, including tumor-associated macrophages (TAMs), have been implicated in tumor progression. We recently described a lineage of mouse monocytes characterized by expression of the Tie2 angiopoietin receptor and required for the vascularization and growth of several tumor models.
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