产品名称
Anti-Lamin A/C Antibody, clone 14, clone 14, Upstate®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
14, monoclonal
species reactivity
canine, chicken, human, rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... LMNA(4000)
Application
Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect Lamin A/C in human endothelial cells.
This antibody has been reported by an independent laboratory to detect Lamin A/C in human endothelial cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Anti-Lamin A/C Antibody, clone 14 detects level of Lamin A/C & has been published & validated for use in IC & WB.
Biochem/physiol Actions
Recognizes Lamin A, MW ~74 kDa and Lamin C, MW ~65 kDa.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Evaluated by western blot on RIPA lysates from A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected Lamin A/C in 20 μg of RIPA lysates from A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected Lamin A/C in 20 μg of RIPA lysates from A431 cells.
General description
Nuclear lamins are composed of the type V intermediate filament proteins. Often referred to as nucleoskeletal proteins they play a key role in nuclear integrity, positioning of nuclear pores, and overall nuclear size and shape. They also play several key functional roles in the maintenance and propagation of the genome--replication, transcription-- as well as the disassembly and reassembly of the nucleus during cell division. In vitro studies have shown that dimers are the basic building blocks of higher order lamin structures and in low concentrations lamins are distributed throughout the nucleoplasm. In humans, there are two types of lamins: A-type lamins (lamins A and C), found primarily in differentiated cells, and B-type lamins (lamins B1 and B2), found in all nucleated cells. Nuclear lamins are involved in a number of essential nuclear functions, including nuclear envelope assembly and disassembly during cell division, DNA synthesis, transcription, and apoptosis. Nuclear lamins have been found to co-localize with DNA synthesis sites.
~74/65 kDa
Immunogen
Peptide from human lamin A/C corresponding to amino acids 398 to 490.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE481
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 50% storage buffer (20 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 1 mg/mL BSA, 0.09% sodium azide) and 50% glycerol. Store at -20°C.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
Rebecca P Hughey et al.
The Journal of biological chemistry, 279(47), 48491-48494 (2004-10-07)
The epithelial Na+ channel (ENaC) is assembled in the endoplasmic reticulum from three structurally related subunits (alpha, beta, and gamma). Channel maturation within the biosynthetic pathway involves cleavage of the alpha and gamma subunits by furin and processing of N-linked
T Sullivan et al.
The Journal of cell biology, 147(5), 913-920 (1999-12-01)
The nuclear lamina is a protein meshwork lining the nucleoplasmic face of the inner nuclear membrane and represents an important determinant of interphase nuclear architecture. Its major components are the A- and B-type lamins. Whereas B-type lamins are found in
Laura D Brown et al.
Endocrinology, 157(6), 2447-2460 (2016-04-07)
Insulin is an important fetal growth factor. However, chronic experimental hyperinsulinemia in the fetus fails to accelerate linear and lean mass growth beyond normal rates. Mechanisms preventing accelerated lean mass accretion during hyperinsulinemia are unknown. To address potential mechanisms, late-gestation
Angelika Oehmig et al.
BMC genomics, 9, 441-441 (2008-09-26)
The identification of novel drug targets by assessing gene functions is most conveniently achieved by high-throughput loss-of-function RNA interference screening. There is a growing need to employ primary cells in such screenings, since they reflect the physiological situation more closely
Sribalasubashini Muralimanoharan et al.
Endocrinology, 159(5), 2022-2033 (2018-03-17)
Dysregulation of human trophoblast invasion and differentiation with placental hypoxia can result in preeclampsia, a hypertensive disorder of pregnancy. Herein, we characterized the role and regulation of miR-1246, which is markedly induced during human syncytiotrophoblast differentiation. miR-1246 targets GSK3β and
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