05-817R-I
Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant
clone 63-1C-8, from rabbit
别名:
H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
63-1C-8, monoclonal
Species reactivity:
human
Application:
DB, FACS, WB
Citations:
10
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
63-1C-8, monoclonal
species reactivity
human
technique(s)
dot blot: suitable, flow cytometry: suitable, western blot: suitable
isotype
IgGκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer10)
Gene Information
human ... HIST1H3F(8968)
General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
~ 17 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to Human Histone H3 phosphorylated at Ser10.
Epitope: Phosphorylated Ser10
Application
Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, is a recombinant rabbit monoclonal antibody that detects Histone H3 phosphorylated at serine 10. This purified recombinant mAb, also known as Anti-H3S10p, is published and validated in WB, Flow Cytometry, and is specificity verified by DB.
Dot Blot Specificity Analysis: AbSurance Histone Antibody Specificity Arrays (cat# 17-668) containing various unmodified and modified Histone peptides (see table) were probed with Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant 0.05ug/mL.
Flow Cytometry Analysis: 0.25 µg/mL of this antibody detected Histone H3 phosphorylated at serine 10 in Jurkat T lymphocytes.
Flow Cytometry Analysis: 0.25 µg/mL of this antibody detected Histone H3 phosphorylated at serine 10 in Jurkat T lymphocytes.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Chromatin Biology
Histones
Chromatin Biology
Biochem/physiol Actions
Recognizes Histone H3 phosphorylated at Serine 10.
Physical form
Format: Purified
Protein A purified
Purified rabbit monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in colcemid treated HeLa cell lysate.
Western Blotting Analysis: 0.05 µg/mL of this antibody detected Histone H3 phosphorylated at serine 10 in 10 µg of colcemid treated HeLa cell lysate.
Western Blotting Analysis: 0.05 µg/mL of this antibody detected Histone H3 phosphorylated at serine 10 in 10 µg of colcemid treated HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Zaleena Akheralie et al.
Development (Cambridge, England), 150(18) (2023-09-08)
The planarian Schmidtea mediterranea is a well-established model of adult regeneration, which is dependent on a large population of adult stem cells called neoblasts. Upon amputation, planarians undergo transcriptional wounding programs and coordinated stem cell proliferation to give rise to
Nicole Lindsay-Mosher et al.
PLoS genetics, 16(2), e1008613-e1008613 (2020-02-23)
The extracellular matrix (ECM) is important for maintaining the boundaries between tissues. This role is particularly critical in the stem cell niche, as pre-neoplastic or cancerous stem cells must pass these boundaries in order to invade into the surrounding tissue.
Regulation of chromatin structure by histone H3S10 phosphorylation.
Johansen, Kristen M and Johansen, J?rgen
Chromosome Research, 14, 393-404 (2006)
M Lucila Scimone et al.
Nature communications, 13(1), 2726-2726 (2022-05-19)
Regeneration often involves the formation of a blastema, an outgrowth or regenerative bud formed at the plane of injury where missing tissues are produced. The mechanisms that trigger blastema formation are therefore fundamental for regeneration. Here, we identify a gene
Casey Kimball et al.
Developmental biology, 457(1), 119-127 (2019-09-27)
Named for its assembly near exon-exon junctions during pre-mRNA splicing, the exon junction complex (EJC) regulates multiple aspects of RNA biochemistry, including export of spliced mRNAs from the nucleus and translation. Transcriptome analyses have revealed broad EJC occupancy of spliced
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