Anti-RIG-I (NT) Antibody

Retinoic acid-inducible gene 1 protein (RIG-1) is a cytoplasmic DExD/H box RNA helicase belonging to the helicase family and containing 2 CARD domains within the N-terminus, a repressor domain (RD), a helicase ATP-binding domain and a helicase C-terminal binding domain. RIG-1 is able to bind the 5’-triphosphate RNA of single or double-stranded viral RNA. After recognizing and binding viral RNA, RIG-1 protein initializes type I IFN production, and within fibroblasts and dendritic cells plays a central role in the regulation of type I IFN production in response to viral presence. There are four known isoforms resulting from alternative splice variants.

~102 kDa observed

Epitope: N-terminus

GST-tagged recombinant protein corresponding to the N-terminus of human RIG-I.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Paz, S., et al. (2011). Cell Research. 1-16.)

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Use Anti-RIG-I (N-Terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect RIG-I (N-Terminus) also known as Retinoic acid-inducible gene 1 protein.

Other homologies: Rhesus Monkey (99% sequence homology).

This antibody recognizes RIG-I at the N-terminus.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
SeV treated and untreated A549 cell lysates

Evaluated by Western Blot in SeV treated and untreated A549 cell lysates.

Western Blot Analysis: 0.05 µg/mL of this antibody detected RIG-I on 10 µg of SeV untreated and treated A549 cell lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.