06-1295
Anti-CDT1 Antibody
serum, from rabbit
别名:
DNA replication factor, Double parked homolog, Double parked, Drosophila, homolog of, chromatin licensing and DNA replication factor 1
生物来源
rabbit
偶联物
unconjugated
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
mouse, rat, human
技术
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... CDT1(81620)
mouse ... Cdt1(67177)
rat ... Cdt1(292071)
一般描述
CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).
~60 kDa
免疫原
KLH-conjugated linear peptide corresponding to human CDT1.
应用
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-CDT1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect CDT1 also known as DNA replication factor, Double parked homolog.
Western Blot Analysis: A 1:1,000 dilution from a previous lot detected CDT1 in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, mouse brain, NIH/3T3, PC3, and RAW264.7 cell lysates.
生化/生理作用
This antibody recognizes CDT1.
外形
Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
制备说明
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
HeLa cell lysate
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected CDT1 in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected CDT1 in 10 µg of HeLa cell lysate.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
Chantal M Maghames et al.
Nature communications, 9(1), 4376-4376 (2018-10-24)
Spatial management of stress-induced protein aggregation is an integral part of the proteostasis network. Protein modification by the ubiquitin-like molecule NEDD8 increases upon proteotoxic stress and it is characterised by the formation of hybrid NEDD8/ubiquitin conjugates. However, the biological significance
Suzanne A Hartford et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(43), 17702-17707 (2011-10-12)
Effective DNA replication is critical to the health and reproductive success of organisms. The six MCM2-7 proteins, which form the replicative helicase, are essential for high-fidelity replication of the genome. Many eukaryotes have a divergent paralog, MCM9, that was reported
Julia I Toth et al.
Cell reports, 1(4), 309-316 (2012-07-27)
Inhibition of NEDD8-activating enzyme (NAE) has emerged as a highly promising approach to treat cancer through the adenosine sulfamate analog MLN4924. Here, we show that selective pressure results in HCT116 colorectal carcinoma cells with decreased MLN4924 sensitivity and identify a
Richard O Adeyemi et al.
PLoS pathogens, 10(4), e1004055-e1004055 (2014-04-05)
Infection by the autonomous parvovirus minute virus of mice (MVM) induces a vigorous DNA damage response in host cells which it utilizes for its efficient replication. Although p53 remains activated, p21 protein levels remain low throughout the course of infection.
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