06-1306
Anti-Mi-2b (CHD4) Antibody
serum, from rabbit
别名:
ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, chromodomain helicase DNA binding protein 4
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
WB
Citations:
2
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CHD4(1108)
General description
217 kDa
CHD-4 is also often referred to as Mi2-beta. This protein is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. This interaction which depends on sumoylation of KLF1 leads to its transcriptional repression.
Immunogen
Epitope: N-terminus
GST-tagged recombinant protein corresponding to mouse CHD-4 at and around the N-terminus.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Use Anti-Mi-2b (CHD4) Antibody (rabbit polyclonal antibody) validated in WB to detect Mi-2b (CHD4) also known as ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, chromodomain helicase DNA binding protein 4.
Biochem/physiol Actions
Rat (96%).
This antibody recognizes CHD-4 at the N-terminus.
Physical form
Unpurified
Unpurified rabbit polyclonal in serum with 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa nuclear extract cell lysate
HeLa nuclear extract cell lysate
Evaluated by Western Blot in HeLa nuclear extract cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected CHD-4 on 10 µg of HeLa nuclear extract cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected CHD-4 on 10 µg of HeLa nuclear extract cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 1
Xiaofei Yu et al.
Haematologica, 104(12), 2361-2371 (2019-04-21)
As high fetal hemoglobin levels ameliorate the underlying pathophysiological defects in sickle cell anemia and beta (β)-thalassemia, understanding the mechanisms that enforce silencing of fetal hemoglobin postnatally offers the promise of effective molecular therapy. Depletion of the Nucleosome Remodeling and
Mario Torrado et al.
The FEBS journal, 284(24), 4216-4232 (2017-10-25)
The nucleosome remodelling and deacetylase (NuRD) complex is essential for the development of complex animals. NuRD has roles in regulating gene expression and repairing damaged DNA. The complex comprises at least six proteins with two or more paralogues of each
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