217 kDa

CHD-4 is also often referred to as Mi2-beta. This protein is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. This interaction which depends on sumoylation of KLF1 leads to its transcriptional repression.

Rat (96%).

This antibody recognizes CHD-4 at the N-terminus.

Epitope: N-terminus

GST-tagged recombinant protein corresponding to mouse CHD-4 at and around the N-terminus.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Use Anti-Mi-2b (CHD4) Antibody (rabbit polyclonal antibody) validated in WB to detect Mi-2b (CHD4) also known as ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, chromodomain helicase DNA binding protein 4.

Unpurified

Unpurified rabbit polyclonal in serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HeLa nuclear extract cell lysate

Evaluated by Western Blot in HeLa nuclear extract cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected CHD-4 on 10 µg of HeLa nuclear extract cell lysate.

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