Anti-SRp30b (SC35) Antibody

SRp30b, also known as SC35, and Splicing factor, arginine/serine-rich 2 (SFRS2) is an essential splicing factor. This protein is encoded by the SFRS2 gene and is a member of the splicing factor SR family. SRp30b is involved in pre-mRNA splicing and is found in bodies in the nucleus that are highly enriched in poly(A) RNA. These nuclear bodies are referred to as speckles, SC 35 domains, or splicing factor compartments (SFCs). The purpose of these bodies is not completely characterized. Speckles correspond largely to, if not entirely to, ultra structures called inter-chromatin granule clusters (IGCs). SRp30b is required for pre-mRNA splicing events and is highly involved in spliceosomal interaction and assembly. This SR protein is necessary for assembly of the ATP-dependent splicing complex. It is also necessary for the ATP-dependent communication between pre-mRNA and U1 and U2 snRNPs.

~35 kDa observed. Uncharacterized bands may be observed at ~10, 58, and 78 kDa

Linear peptide corresponding to human SRp30b.

Anti-SRp30b (SC35) Antibody is a Rabbit Polyclonal Antibody for detection of SRp30b (SC35) also known as serine/arginine-rich splicing factor 2, Splicing factor SC35 & has been validated in WB, ICC.

Western Blot Analysis:
A 1:500 dilution of a representative lot of this antibody detected SRp30b in 10 µg of HeLa cell lysate.

Immunoprecipitation Analysis:
A representative lot of this antibody was used by an independent laboratory in IP. (Jiang, K., et al. (2009). Endocrinology. 150(5):2087-2097.)

Format: Purified

Control
NIH/3T3 cells.

Evaluated by Immunocytochemistry Analysis:
A 1:500 dilution of this antibody detected SRp30b in NIH/3T3 cells.