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Merck
CN

06-157

Anti-RasGAP Antibody

Upstate®, from rabbit

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Species reactivity:
human, rat, mouse, avian
Application:
IP, WB
Citations:
22
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biological source

rabbit

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

Gene Information

human ... TSR3(115939)

species reactivity

human, rat, mouse, avian

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

General description

120kDa

Immunogen

Trp E fusion corresponding to residues 171-448 of human RasGAP

Application

Anti-RasGAP Antibody is an antibody against RasGAP for use in IP & WB.
Research Category
Signaling
Research Sub Category
MAP Kinases

Biochem/physiol Actions

GAP

Physical form

200 μg of protein-A purified IgG. Lyophilized from 200 μl of 0.1 M Tris-HCl, 0.15 M NaCl, and 0.05% Na Azide.
Format: Purified
Protein A chromatography

Preparation Note

Lyophilized: 2 years at -20°C; Rehydrated: 6 months at -20°C Rehydrate in 200 μl of water.

Analysis Note

Western Immunoblot Analysis: This antibody, at 0.5 and 2.0 μg/ml, recognized the 120 kDa GAP protein in a RIPA lysate of human Raji lymphocyte cells, lysates of rat L6 myoblasts and mouse Wehi leukemia cells.
Immunoprecipitation: 5 μg of this antibody immunoprecipitated the 120kDa GAP protein from a RIPA lysate of human Raji lymphocyte cells.

Other Notes

Replaces: 04-577; 04-1039

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

存储类别

11 - Combustible Solids

wgk

WGK 1


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S J Taylor et al.
The Journal of biological chemistry, 270(17), 10120-10124 (1995-04-28)
The c-Src tyrosine kinase phosphorylates and binds to a 68-kDa RNA-binding protein in mitotic cells. We have examined the mechanism and functional consequence of the interaction of c-Src with this protein, Sam 68 (Src associated in mitosis, 68 kDa). In
F Authier et al.
The Biochemical journal, 319 ( Pt 1), 149-158 (1996-10-01)
We have investigated the biosynthesis, subcellular location and expression of insulin-degrading enzyme (IDE). a type-I peroxisomal protease, in semi-permeabilized hepatoma cells using pulse-chase experiments, non-denaturing immunoprecipitation protocols and Northern-blot analyses. In HcpG2 cell lysates prepared from cells radiolabelled with Tran[35S]-label
A conserved region of c-Ha-Ras is required for efficient GTPase stimulation by GTPase activating protein but not neurofibromin
Yoder-Hill, J., et al
The Journal of Biological Chemistry, 270, 27615-27621 (1995)
New enhancements for RadioGraphics online: supplemental material and CME.
J C Honeyman et al.
Radiographics : a review publication of the Radiological Society of North America, Inc, 20(4), 905-906 (2000-07-21)
P J Owen-Lynch et al.
The Journal of biological chemistry, 270(11), 5956-5962 (1995-03-17)
A temperature-sensitive mutant of the v-Abl protein has previously been shown to exhibit tyrosine protein kinase activity in Interleukin 3 (IL-3)-dependent IC.DP cells grown at the permissive temperature (32 degrees C) but not at the restrictive temperature (39 degrees C).

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