生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human
种属反应性(根据同源性预测)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
包装
antibody small pack of 25 μL
技术
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
ambient
靶向翻译后修饰
unmodified
基因信息
rat ... Atp1A1(24211)
一般描述
Sodium/potassium-transporting ATPase subunit alpha-3 (UniProt: P06687; also known as EC: 3.6.3.9, Na(+)/K(+) ATPase alpha-3 subunit, Na(+)/K(+) ATPase alpha(III) subunit, Sodium pump subunit alpha-3) is encoded by the Atp1a3 gene (Gene ID: 24213) in rat. Sodium/potassium-transporting ATPase subunit alpha-3 is a multi-pass membrane protein that is exclusive to the brain tissue and is highly expressed in cell bodies and projections of hippocampus. It is responsible for establishing and maintaining the electrochemical gradients of sodium and potassium ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. Sodium/potassium-transporting ATPase subunit alpha-3 is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic component catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Reduced levels of neuronal sodium/ potassium ATPase activity in ATP1A3 heterozygous mice increases their susceptibility to depression-like endophenotypes by chronic variable stress.
~112 kDa observed; 111.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
特异性
This rabbit polyclonal antibody detects Sodium/potassium-transporting ATPase subunit alpha-3 in human, mouse, and rat brain. It targets an epitope with in 25 amino acids from the N-terminal region.
免疫原
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of rat Sodium/potassium-transporting ATPase subunit alpha-3.
应用
Anti-Na+/K+ ATPase alpha-3, Cat. No. 06-172-I, is a highly specific rabbit polyclonal antibody that targets Sodium/potassium-transporting ATPase subunit alpha-3 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250-1,000 dilution of this antibody detected Na+/K+ ATPase alpha-3 in human cerebral cortex and human cardiac muscle tissue.
Research Category
Neuroscience
Neuroscience
外形
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
制备说明
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Evaluated by Western Blotting in adult human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Na+/K+ ATPase alpha-3 in 10 µg of adult human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Na+/K+ ATPase alpha-3 in 10 µg of adult human brain tissue lysate.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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