Anti-phospho-WAVE2 (Ser343) Antibody

Wiskott-Aldrich Syndrome Protein (WASP)-family Protein Member 2 (known as WAVE2) is found in many cells and tissues, with strong expression in peripheral blood leukocytes. WAVE2 is involved in signaling RTKs and small GTPases. As a whole, the WAVE family of proteins have a general function for regulating the actin cytoskeleton in various tissues.

~75 kDa observed.
An uncharacterized band appears at ~103 kDa in some lysates.

Epitope: Phosphorylated Ser343

KLH-conjugated linear peptide corresponding to human WAVE2 phosphorylated at Ser343.

Anti-phospho-WAVE2 (Ser343) Antibody detects level of phospho-WAVE2 (Ser343) & has been published & validated for use in WB.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Western Blot Analysis: A previous lot of this antibody was used to detect endogenous phospho-WAVE2 (Ser343) in COS-7 and HMECs following EGF stimulation ± pretreatment with U0126 (Mendoza, M.C., et al. (2011). Mol Cell. 41(6):661-671).

This antibody recognizes WAVE2 phosphorylated at Ser343.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
COS-7+EGF cell lysates untreated and lambda phosphatase treated.

Evaluated by Western Blot in COS-7+EGF cell lysates untreated and lambda phosphatase-treated.

Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-WAVE2 (Ser343) in 10 µg of COS-7+EGF cell lysates untreated and lambda phosphatase treated.

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