Anti-FHIT

Bis(5′-adenosyl)-triphosphatase (EC 3.6.1.29; UniProt P49789; also known as AP3A hydrolase, AP3Aase, Diadenosine 5′,5′′′-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein) is encoded by the FHIT gene (Gene ID 2722) in human. Common fragile sites (CFSs) are large genomic loci that display chromosomal instability in response to genotoxic or replicative stress. Many CFSs harbor genes that contribute to cancer development and are non-randomly altered in cancers through genomic alteration. The Fragile histidine triad (FHIT) gene at FRA3B is one of the earliest and most frequently altered genes in the majority of human cancers. FHIT protein plays a role in apoptosis and prevention of the epithelial-mesenchymal transition (EMT). A FHIT-upregulated microRNA, miR-30c, has been identified to mediate the suppression of EMT and metastasis by directly targeting metastasis genes Metadherin (MTDH), High-mobility group AT-hook 2 (HMGA2), and the mesenchymal markers, Vimentin (VIM) and Fibronectin (FN1), in human lung cancer. In addition, FHIT protein also plays an important role in maintaining genome stability, loss of FHIT protein function leads to nucleotide imbalance, spontaneous replication stress, and DNA breaks. Because FHIT loss-induced DNA damage is checkpoint-independent, cells accumulate further DNA damage during subsequent cell cycles, accruing global genome instability that could facilitate oncogenic mutation acquisition and expedite clonal expansion.

This rabbit polyclonal antibody targets a C-terminal epitope region outside the HIT domain.

KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human FHIT.

Anti-FHIT, Cat. No. 07-172-I, is a highly specific rabbit polyclonal antibody that targets FHIT and has been tested in Immunoprecipitation and Western Blotting.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipited FHIT from 500 µg of A549 cell lysate.

Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected FHIT in 10 µg of A549 cell lysate.

~17 kDa observed. 16.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Replaces: 07-172

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.