Anti-WNK4 Antibody

WNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis.

~150 kDa observed. .This protein has 3 isoforms produced by alternative splicing with molecular weights of 134, 73 & 126. Hengl, T., et al. (2010). PNAS. 107(13):6052–6057.

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human WNK4.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

This WNK4 antibody is validated for use in WB for the detection of the WNK4 protein.

Other homologies: Rat (94% sequence homology).

This antibody recognizes WNK4 at the N-terminus.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEK293 cell lysate

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected WNK4 on 10 µg of HEK293 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.