Anti-acetyl-Histone H3 (Lys4) Antibody, Alexa Fluor^™ 488 conjugate

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

~15 kDa observed

Epitope: H3K4ac

KLH-conjugated synthetic peptide containing the sequence ...RT[Kac]Q... in which [Kac] corresponds to acetylated Lys4 of human histone H3.

Anti-acetyl-Histone H3 (Lys4) Antibody, Alexa Fluor^™ 488 conjugate is an antibody against histone Lys4 acetylation for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected detected nuclear histone H3 Lys4 acetylation in HeLa and A431 cells.
The unconjugated antibody (Cat. No. 07-539) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, multiplex assay, and Western blotting applications.

Research Category
Epigenetics & Nuclear Function

Broad species cross-reactivity is expected.

Specificity of this polyclonal antibody toward histone H3 Lys4 acetylation was demonstrated by bead-based multiplex assay and immunogen peptide blocking by Western blotting analysis using the unconjugated antibody (Cat. No. 07-539).

Protein A purified.

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys4 acetylation in HepG2 cells.

Concentration: Please refer to lot specific datasheet.

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