09-271
Rac1b抗体
Upstate®, from rabbit
别名:
Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC, WB
Citations:
26
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RAC1(5879)
General description
Ras相关C3肉毒杆菌毒素底物1（UniProt：P63000；又称细胞迁移诱导基因5蛋白、Ras样蛋白TC25、p21-Rac1）在人体内由Rac1（又称TC25、MIG5）基因（基因ID：5879）编码。Rac1是一种与质膜相关的小GTP酶，在活性GTP结合状态和非活性GDP结合状态之间循环。在其活性状态下，它与多种效应蛋白结合以调节细胞反应。已经描述了通过选择性剪接产生的Rac1的两种同工型。Rac1b与Rac1a的不同之处在于，它含有额外的19个氨基酸，存在于乳腺和结直肠等多种肿瘤中。Rac1b的非GEF依赖性GDP/GTP交换迅速，GTP水解受损，后者由GTP酶激活蛋白部分恢复。它可以与PAK的GTP酶结合结构域结合，但不能以GTP依赖性方式与全长PAK结合，这表明插入并不能完全消除效应子的相互作用。同工型B主要从皮肤和肠道上皮组织中鉴定出来。在肿瘤进展的不同阶段，与各自的邻近组织相比，它在结直肠肿瘤中的表达升高。
观察值为〜17 kDa；计算值为23.47 kDa。在一些裂解液中可能会观察到未表征的条带。
Immunogen
KLH偶联线性肽，对应于人Rac1b N端半部分的15个氨基酸。
Application
RAC1b抗体（货号09-271）是一种兔多克隆抗体，可检测Ras相关的C3肉毒杆菌毒素底物1（Rac1b），经测试可用于免疫细胞化学、免疫组化（石蜡切片）和蛋白质印迹。
免疫细胞化学分析：代表性批次的1:500稀释液在HeLa、A431、HUVEC和NIH/3T3细胞中检测到Rac1b。
免疫组化（石蜡切片）分析：代表性批次的1:250稀释液在人结肠癌和人前列腺癌组织切片中检测到 Rac1b。
免疫组化（石蜡切片）分析：代表性批次的1:250稀释液在人结肠癌和人前列腺癌组织切片中检测到 Rac1b。
Biochem/physiol Actions
这种兔多克隆抗体可特异性检测人和鼠细胞中的Rac1b。
Physical form
含0.05%叠氮化钠和30%甘油的PBS中的纯化的兔多克隆抗体。
Analysis Note
通过蛋白质印迹法在MCF7细胞裂解物中进行了评估。
蛋白质印迹分析：该抗体的1:1,000稀释液在MCF7细胞裂解液中检测到Rac1b。
蛋白质印迹分析：该抗体的1:1,000稀释液在MCF7细胞裂解液中检测到Rac1b。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
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