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Merck
CN

09-275

抗磷酸-JAK2（Tyr1007/1008）抗体

from rabbit, purified by affinity chromatography

别名:

Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
pig, mouse
Application:
WB
Citations:
9

主要文件

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biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

pig, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology), human (based on 100% sequence homology), chicken (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology), porcine (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_004963

UniProt accession no.

O60674

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr1007/pTyr1008)

Gene Information

chicken ... Jak2(374199)
frog ... Jak2(100036599)
human ... JAK2(3717)
mouse ... Jak2(16452)
pig ... Jak2(397201)
rat ... Jak2(24514)

相关类别

General description

Janus激酶2（JAK2）是一种普遍存在的非受体酪氨酸激酶，可与多种细胞表面受体和非受体蛋白相互作用，从而影响一系列细胞过程，包括免疫，细胞生长，发育和分化。 JAK2’s受体伴侣包括催乳素，生长激素，血小板生成素和INF（α 和 β）受体。 JAK2在连接这些受体的信号通路与靶基因的激活中起着重要作用。配体结合后，这些受体激活JAK2，诱导JAK2自磷酸化。该活化的JAK2反过来磷酸化这些受体的胞质结构域，为STAT转录因子提供结合位点，STAT转录因子也被JAK2磷酸化，导致STAT蛋白二聚化和易位至细胞核以实现基因转录。JAK2表达和功能异常可能导致骨髓纤维化和骨髓性白血病。
观测值〜130 kDa。在一些裂解液中在〜48、55、65和100 kDa处可以观察到未鉴定的条带。

Immunogen

与（Tyr1007/Tyr1008）处磷酸化的人JAK2相对应的KLH偶联的线性肽。
表位：磷酸化（Tyr1007/Tyr1008）

Application

抗磷酸化-JAK2 (Tyr1007/1008)抗体是针对磷酸化-JAK2 (Tyr1007/1008)的抗体，可用于WB。
研究类别
信号传导

Biochem/physiol Actions

该抗体识别在(Tyr1007/Tyr1008)处磷酸化的JAK2。

Physical form

亲和纯化
纯化的兔多克隆血清溶于含有0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl和0.05％叠氮化钠的缓冲液中。

Preparation Note

自收到之日起，在2-8°C条件下可稳定保存1年。

Analysis Note

对照
未经处理和经LPS/IFN γ处理的RAW264.7细胞裂解液
已通过蛋白质印迹在未经处理和经LPS/IFN γ处理的RAW264.7细胞裂解液中进行了评估。

蛋白质印迹分析：该抗体以1：5,000的稀释度在10 µg 未经处理和经LPS/IFN γ处理的RAW264.7细胞裂解液中检测到JAK2。

Other Notes

替代：04-1098
浓度：请参考批次特异性浓缩物的分析证书。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

分析证书(COA)

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Wei Tong et al.
The Journal of biological chemistry, 281(50), 38930-38940 (2006-10-21)
Janus tyrosine kinase 2 (JAK2) is essential for signaling by the thrombopoietin (TpoR) and erythropoietin (EpoR) receptors. In the absence of JAK2 most EpoR molecules are retained in the endoplasmic reticulum in an Endo H-sensitive form. In contrast, we show
Céline S Nicolas et al.
Neuron, 73(2), 374-390 (2012-01-31)
The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway is involved in many cellular processes, including cell growth and differentiation, immune functions and cancer. It is activated by various cytokines, growth factors, and protein tyrosine kinases (PTKs) and
Linda M Scott et al.
The New England journal of medicine, 356(5), 459-468 (2007-02-03)
The V617F mutation, which causes the substitution of phenylalanine for valine at position 617 of the Janus kinase (JAK) 2 gene (JAK2), is often present in patients with polycythemia vera, essential thrombocythemia, and idiopathic myelofibrosis. However, the molecular basis of
Rama S Kota et al.
The Journal of biological chemistry, 280(35), 31076-31084 (2005-07-05)
Lipoprotein lipase (LPL) is a key enzyme in the hydrolysis of triglyceride-rich lipoproteins. In vascular diseases, such as atherosclerosis, inflammation plays an important role in the pathogenesis of the disease. We examined the role of LPL in modulating tumor necrosis
Han-Chung Lee et al.
Scientific reports, 11(1), 3847-3847 (2021-02-17)
Ruxolitinib is the first janus kinase 1 (JAK1) and JAK2 inhibitor that was approved by the United States Food and Drug Administration (FDA) agency for the treatment of myeloproliferative neoplasms. The drug targets the JAK/STAT signalling pathway, which is critical
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相关内容

JAK/STAT Signaling Research Focus

The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia.

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