09-290
Anti-phospho-AMPK1 (Thr174) Antibody
from rabbit, purified by affinity chromatography
别名:
5′-AMP-activated protein kinase, catalytic alpha-1 chain, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK alpha 1, AMPK alpha-1 chain, protein kinase, AMP-activated, alpha 1 catalytic subunit
产品名称
Anti-phospho-AMPK1 (Thr174) Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, canine
species reactivity (predicted by homology)
chicken, mouse, rat, bovine, dog
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pThr174)
Gene Information
human ... PRKAA1(5562)
mouse ... Hsf3(245525)
Analysis Note
Control
Lambda phosphatase treated and untreated Hek293 cell lysate
Lambda phosphatase treated and untreated Hek293 cell lysate
Evaluated by Western Blot in Lambda phosphatase treated and untreated HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in Lambda phosphatase treated and untreated HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in Lambda phosphatase treated and untreated HEK293 cell lysate.
Application
Anti-phospho-AMPK1 (Thr174) Antibody detects level of phospho-AMPK1 (Thr174) & has been published & validated for use in WB, IH.
Research Category
Signaling
Signaling
Research Sub Category
Kinases & Phosphatases
Kinases & Phosphatases
Western Blot (SNAP ID) Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in HEK293 cell lysate (see page 2).
Biochem/physiol Actions
This antibody recognizes phosphorylated AMPK1 at Thr174.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
60 kDa
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 174 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
Immunogen
KLH-conjugated linear peptide corresponding to phosphorylated human AMPK1 (Thr174).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Maintain refrigerated at 2-8°C for up to 12 months.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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