09-813
抗环指蛋白8抗体
from rabbit, purified by affinity chromatography
别名:
ring finger protein 8, C3HC4-type zinc finger protein, ring finger protein (C3HC4 type) 8, UBC13/UEV-interacting ring finger protein, E3 ubiquitin-protein ligase RNF8
产品名称
抗环指蛋白8抗体, from rabbit, purified by affinity chromatography
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
species reactivity (predicted by homology)
chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RNF8(9025)
Physical form
亲和纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。
Analysis Note
对照
U2OS核提取物
U2OS核提取物
通过蛋白质印迹对U2OS核提取物进行了评估。
蛋白质印迹分析：0.1 µg/mL的该抗体在10 µg U2OS核提取物中检测到环指蛋白8。
蛋白质印迹分析：0.1 µg/mL的该抗体在10 µg U2OS核提取物中检测到环指蛋白8。
Application
使用在WB，ICC中验证的抗环指蛋白8抗体（兔多克隆抗体）检测环指蛋白8，也称为C3HC4型锌指蛋白。
研究子类别
组蛋白修饰蛋白
细胞周期，DNA复制&修复
组蛋白修饰蛋白
细胞周期，DNA复制&修复
研究类别
表观遗传学 & 核功能
表观遗传学 & 核功能
表观遗传学 & 核功能
表观遗传学 & 核功能
蛋白质印迹（SNAP ID）分析：1 µg/mL代表性批次的该抗体在10 µg U2OS核提取物中检测到环指蛋白8。
免疫细胞化学分析： 代表性批次的1:500稀释液该抗体可在NIH/3T3和A431细胞中检测到环指蛋白8。
免疫细胞化学分析： 代表性批次的1:500稀释液该抗体可在NIH/3T3和A431细胞中检测到环指蛋白8。
Biochem/physiol Actions
该抗体可识别环指蛋白8。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
环指蛋白8（RNF8）是属于环（真正有趣的新基因）指组蛋白的DNA结合蛋白。该家族的特征在于含有能够结合两个锌阳离子的Cys3HisCys4氨基酸基序的环指结构域。RNF8包含两个相关结构域，即C末端的特征性环指基序和N末端的FHA结构域，并且是参与修复蛋白组装到受损DNA位点的关键E3泛素蛋白连接酶。DNA双链断裂后，RNF8被ATM磷酸化的MDC1募集到损伤位点，在该位点它起介导H2A和H2AX泛素化的作用，进而促进TPR53BPI的形成和BRCA1的电离辐射诱导灶（IRIF）。RNF8也可能在胞质分裂，纠正有丝分裂的退出以及RXRA介导的转录活性中发挥调节作用。
观测值〜58 kDa。已经描述了两种不同的亚型。亚型1：55.5 kDa和亚型2：11 kDa
Immunogen
一种对应于人环指蛋白8第135-148位氨基酸的线性肽。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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