09-882
Anti-ALKBH3 Antibody
from rabbit, purified by affinity chromatography
别名:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
技术
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
基因信息
human ... ALKBH3(221120)
一般描述
ABH3 is a member of the Fe(II)/2OG-dependent dioxygenase superfamily, which couples substrate oxidation by converting 2-oxoglutarate (2OG) into succinate and CO2. In humans, ABH3 plays a critical role in alkylated DNA/RNA damage repair and in cancer therapy. While ABH3 may repair alkylated RNA, it demonstrates greater preference for the repair of single- over double-stranded alkylated DNA through oxidative demethylation of 1-methyladenine and 3-methylcytosine.
~35 kDa observed
免疫原
Epitope: N-terminus
Histidine-tagged-recombinant protein corresponding to the the N-terminus of human ALKBH3.
应用
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-ALKBH3 Antibody (rabbit polyclonal antibody) validated in WB to detect ALKBH3 also known as Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1.
Western Blot Analysis: A representative lot from an independent laboratory detected ALKBH3 in PC-3 and LNCap whole cell extract. (Dango, S., et al. (2011). Mol Cell. {Epub ahead of print.})
生化/生理作用
Other homologies Orangutan 98% and Rhesus Monkey 97% sequence homology.
This antibody recognizes the N-terminus of ALKBH3.
外形
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
制备说明
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Human prostate tissue lysate
Human prostate tissue lysate
Evaluated by Western Blot in human prostate tissue lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected ALKBH3 in human prostate tissue lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected ALKBH3 in human prostate tissue lysate.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10-13 - German Storage Class 10 to 13
Yuko Ueda et al.
Biochemistry and biophysics reports, 5, 476-481 (2016-02-10)
Human AlkB homolog 3 (ALKBH3), a homolog of the Escherichia coli protein AlkB, demethylates 1-methyladenine and 3-methylcytosine (3-meC) in single-stranded DNA and RNA by oxidative demethylation. Immunohistochemical analyses on clinical cancer specimens and knockdown experiments using RNA interference in vitro
Yuko Ueda et al.
Scientific reports, 7, 42271-42271 (2017-02-17)
The mammalian AlkB homolog (ALKBH) family of proteins possess a 2-oxoglutarate- and Fe(II)-dependent oxygenase domain. A similar domain in the Escherichia coli AlkB protein catalyzes the oxidative demethylation of 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) in both DNA and RNA. AlkB
Qi Li et al.
The Journal of biological chemistry, 291(21), 11083-11093 (2016-03-27)
The AlkB repair enzymes, including Escherichia coli AlkB and two human homologues, ALKBH2 and ALKBH3, are iron(II)- and 2-oxoglutarate-dependent dioxygenases that efficiently repair N(1)-methyladenine and N(3)-methylcytosine methylated DNA damages. The development of small molecule inhibitors of these enzymes has seen
Zhuojia Chen et al.
Nucleic acids research, 47(5), 2533-2545 (2018-12-13)
Transfer RNA is heavily modified and plays a central role in protein synthesis and cellular functions. Here we demonstrate that ALKBH3 is a 1-methyladenosine (m1A) and 3-methylcytidine (m3C) demethylase of tRNA. ALKBH3 can promote cancer cell proliferation, migration and invasion.
Hanqing He et al.
Blood science (Baltimore, Md.), 2(4), 137-143 (2020-10-23)
ALKBH3, a demethylase responsible for demethylating N1-methyladenosine (m1A) in mRNA and N1-methyldeoxyadenosine in single-stranded DNA, plays an important role in DNA repair and cancer cell proliferation. However, its function in hematopoietic stem cells (HSCs) is unknown. In this study, we
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