1.11350
Bactident™ Kovac′s Indole reagent
Available in a practical dropper bottle, for Escherichia coli
别名:
4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent
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关于此项目
NACRES:
NA.84
UNSPSC Code:
41106200
Quality Segment
description
Bactident™ E. coli 1.13303
form
liquid (ready-to-use)
shelf life
210 days
feature
ready-to-use
composition
(n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)
packaging
pkg of (1 x 30 ml, Plastic bottle)
technique(s)
microbe id | specific enzyme detection: suitable
pH
<1 (20 °C in H2O)
transition temp
flash point 36 °C
density
0.92 g/cm3 at 20 °C
application(s)
environmental
food and beverages
storage temp.
2-8°C
suitability
Enterobacter spp., Escherichia coli
General description
Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
Application
Bactident™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.
Analysis Note
Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.
Other Notes
Replaces: 1.11350.0007
Legal Information
BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany
target_organs
Central nervous system, Respiratory system
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1 - STOT SE 3
存储类别
3 - Flammable liquids
flash_point_f
96.8 °F
flash_point_c
36 °C