方案
≥98% (HPLC)
表单
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated (hygroscopic)
protect from light
颜色
burnt orange
溶解性
DMF: 1 mg/mL
H2O: 1 mg/mL
ethanol: 1 mg/mL
运输
ambient
储存温度
15-25°C
InChI
1S/C17H19N3.ClH/c1-19(2)13-8-9-15-12(10-13)11-14-16(18-15)6-5-7-17(14)20(3)4;/h5-11H,1-4H3;1H
InChI key
ASKSWIKIIFUEOI-UHFFFAOYSA-N
一般描述
A cell-permeable fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, this cationic dye is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognostication of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry providing information about the in situ content, molecular structure, conformation, and environment of many nucleic acid-containing cell constituents.
A cell-permeable, cationic fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognosis of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry that can be used to provide information about the in situ content, molecular structure, and conformation of many nucleic acid-containing cell constituents.
生化/生理作用
Cell permeable: yes
Primary Target
Interacts with DNA and RNA
Interacts with DNA and RNA
Product does not compete with ATP.
制备说明
Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 1 month at 4°C.
其他说明
Gonzalez, K., et al. 1995. Curr. Eye Res.14, 269.
Muro-Cacho, C.A., et al. 1995. J. Immunol.154, 5555.
Olivier, R. 1995. Methods Enzymol.251, 270.
Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA91, 9165.
Darzynkiewicz, Z. 1994. Methods Cell. Biol.41, 401.
Delic, J., et al. 1991. Exp. Cell Res.194, 147.
El-Naggar, A.K., et al. 1991. Cytometry12, 330.
Lopez, F., et al. 1991. Cytometry12, 42.
Gurrieri, S., et al. 1990. Biochemistry29, 3396.
Hermansen, D.K., et al. 1989. Cytometry10, 739.
Muro-Cacho, C.A., et al. 1995. J. Immunol.154, 5555.
Olivier, R. 1995. Methods Enzymol.251, 270.
Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA91, 9165.
Darzynkiewicz, Z. 1994. Methods Cell. Biol.41, 401.
Delic, J., et al. 1991. Exp. Cell Res.194, 147.
El-Naggar, A.K., et al. 1991. Cytometry12, 330.
Lopez, F., et al. 1991. Cytometry12, 42.
Gurrieri, S., et al. 1990. Biochemistry29, 3396.
Hermansen, D.K., et al. 1989. Cytometry10, 739.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Harmful & Carcinogenic / Teratogenic (E)
警示用语：
Warning
危险声明
危险分类
Muta. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
此项目有
Simultaneous analysis of cellular RNA and DNA content.
Z Darzynkiewicz
Methods in cell biology, 41, 401-420 (1994-01-01)
D K Hermansen et al.
Cytometry, 10(6), 739-742 (1989-11-01)
The sensitivity of bladder wash flow cytometry (BWFCM), voided urinary cytology (VUC), and cytology of catheterized urine obtained at the time of cystoscopy (CUC) were reviewed on all women evaluated for bladder cancer at Memorial Sloan-Kettering Cancer Center between June
G L Busch et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(19), 9165-9169 (1994-09-13)
Cell swelling is shown to induce an increase in acridine orange fluorescence intensity, an effect pointing to the alkalinization of acidic vesicles. Since autophagic hepatic proteolysis is accomplished by pH-sensitive proteinases within acidic lysosomes, this effect may contribute to the
F Lopez et al.
Cytometry, 12(1), 42-49 (1991-01-01)
The antibody Ki67 is currently used to evaluate the proliferative fraction of solid tumors and some hematological malignancies. We have used phytohemagglutinin (PHA)-stimulated peripheral blood lymphocytes as a model to study the entry of quiescent cells into cell cycle and
K Gonzalez et al.
Current eye research, 14(4), 269-273 (1995-04-01)
The lens epithelial cells are a primary site of involvement in galactosemia. Changes in their size, shape and proliferative capacity have been observed upon exposure to high galactose. In this report, changes in the cell cycle pattern of normal and
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