biological source
human
recombinant
expressed in baculovirus infected Sf21 cells
mol wt
Mw 62 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
Quality Level
General description
Product Source: N-terminal 6His-tagged recombinant human full-length ULK3. Expressed by baculovirus in Sf21 insect cells.
Biochem/physiol Actions
Protein Target: ULK3
Target Sub-Family: Misc
Physical form
50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.
Ni2+/NTA-agarose
Preparation Note
Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Analysis Note
Routinely evaluated by phosphorylation of casein.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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