16-210
Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate
clone 2F3.2, Upstate®, from mouse
别名:
70 kDa zeta-associated protein, Syk-related tyrosine kinase, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD
一般描述
70 kDa
Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals
Product Description: Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC)
Immunogen: GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 corresponding to amino acids 1-254
Product Description: Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC)
Immunogen: GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 corresponding to amino acids 1-254
ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
免疫原
GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 correspon-ding to amino acids 1-254. Clone 2F3.2.
应用
Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate detects level of ZAP-70 & has been published & validated for use in FC, IC & WB.
Immunocytochemistry:
5-20 µg/mL of a previous lot detected ZAP-70 in Jurkat cells fixed with formaldehyde and permeabilized with saponin.
5-20 µg/mL of a previous lot detected ZAP-70 in Jurkat cells fixed with formaldehyde and permeabilized with saponin.
Research Category
Signaling
Signaling
Research Sub Category
Immunological Signaling
Immunological Signaling
生化/生理作用
Expected to cross-react with rat.
Recognizes human ZAP-70.
外形
Protein G Purified
Mouse monoclonal FITC-conjugated IgG2a in buffer containing PBS containing 0.05% sodium azide.
Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC). Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.
Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC). Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.
制备说明
Stable for 6 months at 2-8°C from date of receipt. For maximum recovery of product, centrifuge original vial prior to removing cap.
分析说明
Control
Western Blot: Jurkat cell lysate.
Western Blot: Jurkat cell lysate.
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis:
1:500 dilution of this lot detected ZAP 70 on 10 μg of Jurkat lysates.
Western Blot Analysis:
1:500 dilution of this lot detected ZAP 70 on 10 μg of Jurkat lysates.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Fortunato Morabito et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 19(21), 5890-5900 (2013-09-17)
To investigate the incidence and clinical relevance of classic and new prognostic markers, IGHV gene mutational status, and chromosomal abnormalities in clinical monoclonal B lymphocytosis (cMBL) compared with Rai stage 0 chronic lymphocytic leukemia (Rai0-CLL). A group of 136 patients
TCR signaling: another Abl-bodied kinase joins the cascade.
Wange, Ronald L
Current Biology, 14, R562-R564 (2004)
M Szabo et al.
International immunology, 24(2), 79-87 (2011-12-31)
Zeta-chain-associated protein kinase of 70kDa (ZAP-70) kinase is a key regulator in the early steps of TCR signaling but some aspects of its fine regulation are still unclear. From its 31 tyrosine (Y) residues, 11 phosphorylation sites have been identified
Chronic lymphocytic leukaemia: new biological markers for assessing prognosis.
Wagner, Simon D and Cwynarski, Kate
The Hematology Journal : The Official Journal of the European Haematology Association / EHA, 5, 197-201 (2004)
Roberta Pascolutti et al.
Cell reports, 27(10), 3049-3061 (2019-06-06)
Adaptor protein 2 (AP2) is a major constituent of clathrin-coated pits (CCPs). Whether it is essential for all forms of clathrin-mediated endocytosis (CME) in mammalian cells is an open issue. Here, we demonstrate, by live TIRF microscopy, the existence of
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