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Merck
CN

17-1010

Magna ChIP®-Seq ChIP and NGS Library Preparation Kit

chromatin IP & next generation sequencing library preparation from genomic DNA.

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NACRES:
NA.84
UNSPSC Code:
41105331

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manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable, sample preparation: suitable

Quality Level

100

General description

ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP-Seq has become an indispensable tool. The Magna ChIP-Seq Kit simplifies this technique by providing a validated set of reagents, easy-to-follow protocols, controls, and in process quality control guidelines to allow virtually any laboratory to conduct genome-wide analysis of DNA-protein interactions and the profiling of epigenetic marks.

Application

Magna ChIP-Seq Chromatin IP & Next Generation Sequencing Library Preparation Kit containing all key reagents for chromatin IP & next generation sequencing library preparation from genomic DNA.

Features and Benefits

  • Effective ChIP protocol simplifies production and immunoprecipitation of high quality chromatin
  • Reliable ChIP-Seq library construction from as little as 1 ng of purified ChIP DNA
  • Easy-to-follow protocols and in-process quality control guidelines help ensure successful ChIP and library construction in as little as 2 days.
  • Magna ChIP A+G bead blend allows the use of antibodies from virtually any species or class.
  • Enzymes and buffers provided in convenient master mix formulations streamlines library construction
  • Validate the ChIP procedure using inlcuded positive and negative control antibodies and a control primer set.
  • Proven performance through construction and sequencing of genomic DNA libraries on an Illumina Genome Analyzer II

Packaging

  • 10 library constructions.
  • Three modules containing all key reagents for chromatin immunoprecipitation and next generation sequencing library preparation from genomic DNA.

Preparation Note

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.

Other Notes

  • Magna ChIP A/G (2° to 8°C) -Chromatin IP Reagents
  • Magna ChIP A/G (-20°C) -Chromatin IP Reagents
  • Magna ChIP-Seq Library Construction (-20°C) Reagents

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

存储类别

10 - Combustible liquids

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相关内容

An Introduction to Antibodies and Their Applications

The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).

Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals

Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA- and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. In a typical ChIP experiment either protein A or G is selected for enrichment depending on the antibody isotype. However, proteins A and G possess differing affinities for human and mouse IgGs. Complicating this choice, for some antibody isotypes there is affinity for both protein A and G. In addition, we have observed that independent of the isotype the affinity of a specific antibody for protein A or G can vary depending on the specific clone, purification method, and source.

Magna ChIP-Seq™ Kit

Genome-wide mapping of DNA-protein interactions and epigenetic marks using chromatin immunoprecipitation combined with next generation sequencing (ChIP-Seq) is indispensable for many gene regulation studies. The Magna ChIPSeq Kit simplifies this technique, enabling the performance of ChIP-Seq by virtually any laboratory.

Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

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