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Merck
CN

17-648

ChIPAb+ 二甲基组蛋白H3（Lys9）- ChIP经验证的抗体和引物组

serum, from rabbit

别名:

H3K9me2, Histone H3 (di methyl K9), Histone H3K9me2

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UNSPSC Code:
12352203
NACRES:
NA.52
eCl@ss:
32160702

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产品名称

ChIPAb+ 二甲基组蛋白H3（Lys9）- ChIP经验证的抗体和引物组, serum, from rabbit

biological source

rabbit

antibody form

serum

clone

polyclonal

species reactivity

human, mouse

species reactivity (predicted by homology)

mammals

manufacturer/tradename

ChIPAb+
Upstate®

technique(s)

ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NM_003493

UniProt accession no.

Q66I33

shipped in

dry ice

Quality Level

100

Gene Information

human ... H3F3B(3021)

相关类别

Analysis Note

对照
包括阴性对照抗体正常兔血清和对人β-珠蛋白启动子特异性的对照引物。
染色质免疫沉淀：
使用4 μL正常兔抗血清或4 μL抗二甲基组蛋白H3（Lys9）血清和Magna ChIP A（目录号17-610）试剂盒，对未经处理的HeLa细胞（1 X 106细胞当量）制备的超声染色质进行染色质免疫沉淀。
通过qPCR，使用β-珠蛋白人启动子侧翼的对照ChIP引物，验证了二甲基组蛋白H3（Lys9）相关DNA片段的成功免疫沉淀（请参见图）。

Application

二甲基组蛋白H3（Lys9）ChIP验证的抗体&引物组，包括ChIP级抗体 & 特异性对照PCR引物用于H3K9Me2染色质免疫沉淀。
染色质免疫沉淀：
使用4 μL正常兔抗血清或抗二甲基组蛋白H3（Lys9）血清和Magna ChIP A试剂盒（目录号17-610），对从未处理HeLa细胞制备的超声染色质（1 X 106细胞当量）进行染色质免疫沉淀（请参见图）。使用位于人β-珠蛋白启动子侧翼的β-珠蛋白ChIP引物或扩增人GAPDH启动子的引物，通过qPCR验证了二甲基组蛋白H3（Lys9）相关DNA片段的成功免疫沉淀，该启动子在HeLa细胞中无转录活性。显示了每个qPCR引物组相对于标准曲线的输入百分比。
请参阅EZ-Magna G ChIP（目录号17-408）或EZ-ChIP（目录号17-371）方案以获取实验详细信息。

蛋白质印迹分析和肽抑制：
HeLa酸提取物通过电泳分离，转移到硝酸纤维素膜上，并用抗二甲基组蛋白H3（Lys9）（1:500，泳道1）探测，或与0.4 μM组蛋白H3肽预孵育，并进行以下修饰：
泳道2：线性未修饰
泳道3：分支未修饰
泳道4：分支三甲基
泳道5：线性三甲基
泳道6：分支二甲基
泳道7：线性二甲基
泳道8：分支单甲基
泳道9：线性单甲基
使用与HRP偶联的山羊抗–兔二抗和化学发光检测系统观察蛋白质
研究子类别
染色质生物学
研究类别
表观遗传学&核功能

Biochem/physiol Actions

二甲基组蛋白H3（Lys9）

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

17 kDa
对每批、每次的全部ChIPAb+抗体的染色质沉淀进行单独验证。 每个ChIPAb+抗体组均包含对照引物（通过qPCR对每个批次进行检测），以在基因座特异性环境下对IP结果进行生物学验证。提供了qPCR方案和引物序列，使研究人员能够在染色质环境中使用我们的抗体时验证ChIP方案。每组还包括阴性对照抗体，以确保ChIP反应的特异性。
ChIPAb+二甲基组蛋白H3（Lys9）组包括抗二甲基组蛋白H3（Lys9）抗体、阴性对照抗体（正常兔血清）和qPCR引物，其可扩增人β珠蛋白基因启动子内的110 bp区域。 二甲基组蛋白H3（Lys9）和阴性对照抗体以可扩展的“每个ChIP”反应大小提供，可用于功能上验证二甲基组蛋白H3（Lys9）相关染色质的沉淀。
组蛋白的甲基化可以发生在两个不同的残基上：精氨酸或赖氨酸。组蛋白甲基化可以与转录激活或抑制相关，具体取决于甲基化残基。组蛋白H3的赖氨酸9可以通过不同的组蛋白甲基转移酶（HMTs）（例如，SuvH39H1或G9a）单甲基化、二甲基化或三甲基化。该甲基化的赖氨酸可以通过组蛋白脱甲基酶（如JMJD1A，LSD1或JMJD2C）脱甲基。该残基的甲基化主要与转录抑制有关。

Immunogen

二甲基组蛋白H3（Lys9）兔血清是针对含有序列..Rme2KSTG..的KLH-偶联分支合成肽制备的，其中me2K对应于人组蛋白H3残基9的二甲基赖氨酸
表位：二甲基Lys9

Other Notes

抗二甲基组蛋白H3（Lys9）（兔血清），1小瓶

阴性ChIP对照血清，1小瓶

ChIP引物β-珠蛋白，1小瓶

Packaging

25次测定/试剂盒，约4 μL/染色质免疫沉淀

Physical form

二甲基组蛋白H3（Lys9）（兔多克隆血清）。 一个小瓶含有100 μL抗血清（含0.05%叠氮化钠）。

正常兔血清。一个小瓶含有100 uL抗血清（含0.05%叠氮化钠）。

ChIP引物，β-珠蛋白。一个小瓶包含75 μL的5 μM人β-珠蛋白特异性引物。
用于：AGG ACA GGT ACG GCT GTC ATC
REV: TTT ATG CCC AGC CCT GGC TC

Preparation Note

自收到之日起在-20°C可稳定保存1年

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

10 - Combustible liquids

法规信息

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Epigenetic silencing of tumor suppressor genes commonly occurs in human cancers via increasing DNA methylation and repressive histone modifications at gene promoters. However, little is known about how pathogenic environmental factors contribute to cancer development by affecting epigenetic regulatory mechanisms.
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Tumor-repopulating cells (TRCs) are a tumorigenic sub-population of cancer cells that drives tumorigenesis. We have recently reported that soft fibrin matrices maintain TRC growth by promoting histone 3 lysine 9 (H3K9) demethylation and Sox2 expression and that Cdc42 expression influences
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