Anthrax Lethal Factor, Recombinant, Bacillus anthracis, B. anthracis - Calbiochem

Recombinant, Bacillus anthracis anthrax lethal factor expressed in a special strain of B. anthracis. One of three protein components of anthrax toxin produced by the pathogenic bacterium, Bacillus anthracis. Highly specific protease component that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family. LF is comprised of four domains: domain I that binds to protective antigen (necessary for entry into the cellular target) and domains II, III, and IV which form a long groove to hold and cleave the target proteins. Requires passage through an acidic vesicle prior to translocation to the cytoplasm of the cell. Cleavage of target proteins leads to the inhibition of multiple signaling pathways and cell death.

Primary Target
MAPKK

yes

Lyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.

Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.

Please refer to the vial label for lot-specific reconstitution volume.

Single major band by SDS-PAGE

Brossier, F., and Mock, M. 2001. Toxicon39, 1747.
Friedlander, A.M. 2001. Nature414, 160.
Gupta, P., et al. 2001. Biochem. Biophys. Res. Commun.284, 568.
Pannifer, A.D., et al. 2001. Nature414, 160.
Pugsley, A.P. 1996. Proc. Natl. Acad. Sci. USA93, 8155.
Leppla, S.H. 1991. Methods Enzymol.195, 153.

One unit is defined as the amount of enzyme required to catalyze the release of 1.0 pmole of cleaved MAPKKide substrate per min at 37°C, in 20 mM HEPES, pH 8.2.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)