176904
Anthrax Lethal Factor Protease Substrate III, Fluorogenic
An N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax lethal factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM.
别名:
Anthrax Lethal Factor Protease Substrate III, Fluorogenic, Anthrax LF Protease Substrate III, Fluorogenic, Bacillus anthracis LF Protease Substrate III, Fluorogenic
assay
≥97% (HPLC)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
solubility
water: 1 mg/mL, 5% acetic acid: 5 mg/mL, DMSO: 5 mg/mL
fluorescence
λex ~360 nm, λem ~460 nm
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
An N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax Lethal Factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM. Reported to be useful for high-throughput screening of LF inhibitors. Excitation max.: ~360 nm; emission max.: ~460 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Measuring Anthrax lethal factor (LF) metalloproteolytic activity
Measuring Anthrax lethal factor (LF) metalloproteolytic activity
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Ac-Gly-Tyr-βAla-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Val-Leu-Arg-AMC
Tonello, F., et. al. 2002. Nature418, 386.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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