Betacellulin, Mouse, Recombinant

Recombinant, mouse betacellulin (BTC) cinsisting of amino acids 32-118 and expressed in E. coli. Mouse BTC is 93% identical to the amino acid sequence of rat and 79% with that of human. BTC, a heparin-binding protein, is expressed in tissues such as uterus, liver, pancreas, and kidney and promotes pancreatic beta-cell growth and differentiation. Also known as a potent mitogen for retinal pigment epithelial cells, fibroblasts, and vascular smooth muscle cells.

ED₅₀ = 0.15-0.6 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Bastian, S.E., et al. 2001. J. Endocrinol.168, 203.
Li, L., et al. 2001. Endocrinology142, 5379.
Dunbar, A.J. and C. Goddard. 2000. Int. J. Biochem. Cell Biol.32, B805.
Tada, H., et al. 1999. J. Cell Biochem.72, 423.
Pathak, B.G., et al. 1995. Genomics28, 116.
Shing, Y., et al. 1993. Science259, 1604.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)