biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
28F3, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, bovine
should not react with
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
ambient
storage temp.
−20°C
General description
Purified mouse monoclonal antibody. Recognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2).
Recognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2). Recognizes both native and denatured forms of the small subunit of calpain.
This Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Calpain-1/2, Small Subunit.
Immunogen
Human Placenta
purified calpain from human placenta
Application
ELISA (1:2000)
Immunoblotting (1:2000)
Immunoprecipitation (1:500)
Immunoblotting (1:2000)
Immunoprecipitation (1:500)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM sodium chloride, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
MDBK cells
MDBK cells
Other Notes
Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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